ArticlePloS one2014
Covalent and density-controlled surface immobilization of E-cadherin for adhesion force spectroscopy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- STED/AFM as a tool to investigate mechanical and adhesive properties of migrating keratinocytes.Cell adhesion & migration · 2026Article
- Cadherins and catenins in cancer: connecting cancer pathways and tumor microenvironment.Frontiers in cell and developmental biology · 2023Review
- N-Cadherin adhesive ligation regulates mechanosensitive neural stem cell lineage commitment in 3D matrices.Biomaterials science · 2022Article
- Nucleation of cadherin clusters on cell-cell interfaces.Scientific reports · 2022Article
- Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension.Nature cell biology · 2022Article
- Tuning Epithelial Cell-Cell Adhesion and Collective Dynamics with Functional DNA-E-Cadherin Hybrid Linkers.Nano letters · 2022Article
- Advancing cell instructive biomaterials through increased understanding of cell receptor spacing and material surface functionalization.Regenerative engineering and translational medicine · 2021Article
- A Framework of Paracellular Transport via Nanoparticles-Induced Endothelial Leakiness.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Single-molecule force spectroscopy reveals the dynamic strength of the hair-cell tip-link connection.Nature communications · 2021Article
- Article
- Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish.Histochemistry and cell biology · 2020Article
- Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting.ACS applied materials & interfaces · 2020Article
- Controlling Fibronectin Fibrillogenesis Using Visible Light.Frontiers in molecular biosciences · 2020Article
- Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes.Frontiers in molecular biosciences · 2020Review
- Microfluidic device performing on flow study of serial cell-cell interactions of two cell populations.RSC advances · 2019Article
- How Do We Know when Single-Molecule Force Spectroscopy Really Tests Single Bonds?Biophysical journal · 2018Review
- N-cadherin adhesive interactions modulate matrix mechanosensing and fate commitment of mesenchymal stem cells.Nature materials · 2016Article
- Recent advances in covalent, site-specific protein immobilization.F1000Research · 2016Review
- Cell adhesion and urothelial bladder cancer: the role of cadherin switching and related phenomena.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Energy Landscape of Alginate-Epimerase Interactions Assessed by Optical Tweezers and Atomic Force Microscopy.PloS one · 2015Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
E-cadherin is a key cell-cell adhesion molecule but the impact of receptor density and the precise contribution of individual cadherin ectodomains in promoting cell adhesion are only incompletely understood. Investigating these mechanisms would benefit from artificial adhesion substrates carrying different cadherin ectodomains at defined surface density. We therefore developed a quantitative E-cadherin surface immobilization protocol based on the SNAP-tag technique. Extracellular (EC) fragments of E-cadherin fused to the SNAP-tag were covalently bound to self-assembled monolayers (SAM) of thiols carrying benzylguanine (BG) head groups. The adhesive functionality of the different E-cadherin surfaces was then assessed using cell spreading assays and single-cell (SCSF) and single-molecule (SMSF) force spectroscopy. We demonstrate that an E-cadherin construct containing only the first and second outmost EC domain (E1-2) is not sufficient for mediating cell adhesion and yields only low single cadherin-cadherin adhesion forces. In contrast, a construct containing all five EC domains (E1-5) efficiently promotes cell spreading and generates strong single cadherin and cell adhesion forces. By varying the concentration of BG head groups within the SAM we determined a lateral distance of 5-11 nm for optimal E-cadherin functionality. Integrating the results from SCMS and SMSF experiments furthermore demonstrated that the dissolution of E-cadherin adhesion contacts involves a sequential unbinding of individual cadherin receptors rather than the sudden rupture of larger cadherin receptor clusters. Our method of covalent, oriented and density-controlled E-cadherin immobilization thus provides a novel and versatile platform to study molecular mechanisms underlying cadherin-mediated cell adhesion under defined experimental conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.