ArticlePloS one2014
Metabolic and target-site mechanisms combine to confer strong DDT resistance in Anopheles gambiae.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 155 citations in OpenAlex.
- Design of a study to determine the impact of insecticide resistance on malaria vector control: a multi-country investigation.Malaria journal · 2015Trial
- Synergistic action of different molecular mechanisms causes striking levels of insecticide resistance in the malaria vectorProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex.BMC genomics · 2026Article
- Genomic Signatures of Microgeographic Adaptation in Anopheles coluzzii Across Urban, Rural, and Forested Environments in Gabon.Molecular ecology · 2026Article
- Spatial and temporal signatures of genomic insecticide resistance in the Anopheles arabiensis mosquito malaria vector from Ethiopia.Research square · 2026Article
- Comparative analysis reveals concentration-dependent and site-specific detoxification gene expression patterns in pyrethroid-resistant Anopheles gambiae s.l. from Accra, Ghana.Scientific reports · 2026Article
- Genomic population structure and insecticide resistance mechanisms in the malaria vector An. coluzzii across contrasting bioclimatic zones in West Africa.BMC genomics · 2026Article
- Distinct Genetic Footprint of Adaptation Observed WithinResearch and reports in tropical medicine · 2026Article
- Comparative transcriptomic analysis of pyrethroid-resistant Anopheles gambiae s.l. from Ghana, reveals concentration-dependent and site-specific patterns of gene expression.Research square · 2025Article
- Impact of combined vector control interventions on Anopheles gambiae sensu lato resistance dynamics in a high pyrethroid resistance settings in Southwestern Burkina Faso.Malaria journal · 2025Article
- Genome-wide analysis of genetic diversity in Anopheles darlingi from Rondônia State, Brazil.Communications biology · 2025Article
- Genomic population structure and insecticide resistance mechanisms in the malaria vector An. coluzzii across contrasting bioclimatic zones in West Africa.Research square · 2025Article
- Evaluation of the efficacy of 20% IR3535Parasites & vectors · 2025Article
- Novel insecticide resistance mutations associated with variable PBO synergy in Anopheles gambiae s.l. from the Democratic Republic of Congo.Scientific reports · 2025Article
- An atlas of positive selection in the genomes of major malaria vectors.bioRxiv : the preprint server for biology · 2025Article
- Exploring the metabolic and cuticular mechanisms of increased pyrethroid resistance in Anopheles gambiae S.l populations from Ghana.Scientific reports · 2025Article
- Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae.Parasites & vectors · 2025Article
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- Application of a targeted amplicon sequencing panel to screen for insecticide resistance mutations in Anopheles darlingi populations from Brazil.Scientific reports · 2025Article
- Article
28 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors at 6 institutions in 4 countries.
Funding
Abstract
The development of resistance to insecticides has become a classic exemplar of evolution occurring within human time scales. In this study we demonstrate how resistance to DDT in the major African malaria vector Anopheles gambiae is a result of both target-site resistance mechanisms that have introgressed between incipient species (the M- and S-molecular forms) and allelic variants in a DDT-detoxifying enzyme. Sequencing of the detoxification enzyme, Gste2, from DDT resistant and susceptible strains of An. gambiae, revealed a non-synonymous polymorphism (I114T), proximal to the DDT binding domain, which segregated with strain phenotype. Recombinant protein expression and DDT metabolism analysis revealed that the proteins from the susceptible strain lost activity at higher DDT concentrations, characteristic of substrate inhibition. The effect of I114T on GSTE2 protein structure was explored through X-ray crystallography. The amino acid exchange in the DDT-resistant strain introduced a hydroxyl group nearby the hydrophobic DDT-binding region. The exchange does not result in structural alterations but is predicted to facilitate local dynamics and enzyme activity. Expression of both wild-type and 114T alleles the allele in Drosophila conferred an increase in DDT tolerance. The 114T mutation was significantly associated with DDT resistance in wild caught M-form populations and acts in concert with target-site mutations in the voltage gated sodium channel (Vgsc-1575Y and Vgsc-1014F) to confer extreme levels of DDT resistance in wild caught An. gambiae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.