ArticlePloS one2014
Fusion FISH imaging: single-molecule detection of gene fusion transcripts in situ.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Recent advances in cancer fusion transcript detection.Briefings in bioinformatics · 2023Review
- circCsnk1g3- and circAnkib1-regulated interferon responses in sarcoma promote tumorigenesis by shaping the immune microenvironment.Nature communications · 2022Article
- Consensus Recommendations to Optimize Testing for New Targetable Alterations in Non-Small Cell Lung Cancer.Current oncology (Toronto, Ont.) · 2022Review
- Article
- Article
- Stress granule formation, disassembly, and composition are regulated by alphavirus ADP-ribosylhydrolase activity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Tudor-domain protein TDRD7, mutated in congenital cataract, controls the heat shock protein HSPB1 (HSP27) and lens fiber cell morphology.Human molecular genetics · 2020Article
- Sex Determination by Two Y-Linked Genes in Garden Asparagus.The Plant cell · 2020Article
- Mechanistic Target of Rapamycin Regulates the Oligodendrocyte Cytoskeleton during Myelination.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- EWS-FLI1 modulated alternative splicing of ARID1A reveals novel oncogenic function through the BAF complex.Nucleic acids research · 2019Article
- Fluorescence In Situ Imaging of Dendritic RNAs at Single-Molecule Resolution.Current protocols in neuroscience · 2019Article
- Intragenic antagonistic roles of protein and circRNA in tumorigenesis.Cell research · 2019Article
- Genome-wide colocalization of RNA-DNA interactions and fusion RNA pairs.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- mRNA detection in budding yeast with single fluorophores.Nucleic acids research · 2017Article
- The long noncoding RNAScience advances · 2017Article
- Astrocyte-produced leukemia inhibitory factor expands the neural stem/progenitor pool following perinatal hypoxia-ischemia.Journal of neuroscience research · 2016Article
- Profiling stem cell states in three-dimensional biomaterial niches using high content image informatics.Acta biomaterialia · 2016Article
- Development of RNA-FISH Assay for Detection of Oncogenic FGFR3-TACC3 Fusion Genes in FFPE Samples.PloS one · 2016Article
- Quantitative detection of rare interphase chromosome breaks and translocations by high-throughput imaging.Genome biology · 2015Article
- Localization and abundance analysis of human lncRNAs at single-cell and single-molecule resolution.Genome biology · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Double-stranded DNA breaks occur on a regular basis in the human genome as a consequence of genotoxic stress and errors during replication. Usually these breaks are rapidly and faithfully repaired, but occasionally different chromosomes, or different regions of the same chromosome, are fused to each other. Some of these aberrant chromosomal translocations yield functional recombinant genes, which have been implicated as the cause of a number of lymphomas, leukemias, sarcomas, and solid tumors. Reliable methods are needed for the in situ detection of the transcripts encoded by these recombinant genes. We have developed just such a method, utilizing single-molecule fluorescence in situ hybridization (sm-FISH), in which approximately 50 short fluorescent probes bind to adjacent sites on the same mRNA molecule, rendering each target mRNA molecule visible as a diffraction-limited spot in a fluorescence microscope. Utilizing this method, gene fusion transcripts are detected with two differently colored probe sets, each specific for one of the two recombinant segments of a target mRNA; enabling the fusion transcripts to be seen in the microscope as distinct spots that fluoresce in both colors. We demonstrate this method by detecting the BCR-ABL fusion transcripts that occur in chronic myeloid leukemia cells, and by detecting the EWSR1-FLI1 fusion transcripts that occur in Ewing's sarcoma cells. This technology should pave the way for accurate in situ typing of many cancers that are associated with, or caused by, fusion transcripts.
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