ArticleScientific reports2014
Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed.
- Increased hydrogen and ethanol production in transformants of the filamentous cyanobacterium Phormidium lacuna.Archives of microbiology · 2026Article
- Green metabolic engineering of Synechocystis sp. PCC 6803 for efficient production and sustainable extraction of β-cryptoxanthin and zeaxanthin.Microbial cell factories · 2026Article
- Heterologous Production of the Structurally Complex Diterpenoid Forskolin inBioengineering (Basel, Switzerland) · 2025Article
- Cnb proteins affect the intracellular 2-MIB retention in heterologous gene expression systems.Archives of microbiology · 2025Article
- Greening the Production of Indigo Blue Exploiting Light and a Recombinant Synechocystis sp. PCC6803 Strain Expressing the Enzyme mFMO.Microbial biotechnology · 2025Article
- Co-expression of auxiliary genes enhances the activity of a heterologous OBiotechnology for biofuels and bioproducts · 2025Article
- Retention of a SulP-family bicarbonate transporter in a periplasmic N2-fixing cyanobacterial endosymbiont of an open ocean diatom.The ISME journal · 2025Article
- Identification of acidic stress-responsive genes and acid tolerance engineering in Synechococcus elongatus PCC 7942.Applied microbiology and biotechnology · 2024Article
- Engineering cyanobacteria as a new platform for producing taxol precursors directly from carbon dioxide.Biotechnology for biofuels and bioproducts · 2024Article
- Nutraceutical prospects of genetically engineered cyanobacteria- technological updates and significance.World journal of microbiology & biotechnology · 2024Review
- Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications.Biomimetics (Basel, Switzerland) · 2024Review
- Determinants ofGenes · 2024Article
- Glucosylglycerol phosphorylase, a potential novel pathway of microbial glucosylglycerol catabolism.Applied microbiology and biotechnology · 2024Article
- Promoting Heme and Phycocyanin Biosynthesis inMarine drugs · 2023Article
- Stress response requires an efficient connection between glycogen and central carbon metabolism by phosphoglucomutases in cyanobacteria.Journal of experimental botany · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Engineering plant family TPS into cyanobacterial host for terpenoids production.Plant cell reports · 2022Review
- Insights into cyanobacterial alkane biosynthesis.Journal of industrial microbiology & biotechnology · 2022Review
- Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria.Biotechnology for biofuels · 2021Review
6 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
Cyanobacteria are oxygenic photosynthetic prokaryotes that play important roles in the global carbon cycle. Recently, engineered cyanobacteria capable of producing various small molecules from CO2 have been developed. However, cyanobacteria are seldom considered as factories for producing proteins, mainly because of the lack of efficient strong promoters. Here, we report the discovery and verification of a super-strong promoter P(cpc560), which contains two predicted promoters and 14 predicted transcription factor binding sites (TFBSs). Using P(cpc560), functional proteins were produced at a level of up to 15% of total soluble protein in the cyanobacterium Synechocystis sp. 6803, a level comparable to that produced in Escherichia coli. We demonstrated that the presence of multiple TFBSs in P(cpc560) is crucial for its promoter strength. Genetically transformable cyanobacteria neither have endotoxins nor form inclusion bodies; therefore, P(cpc560) opens the possibility to use cyanobacteria as alternative hosts for producing heterogeneous proteins from CO2 and inorganic nutrients.
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