ArticleNature protocols2014
Epitope-tagged protein-based artificial miRNA screens for optimized gene silencing in plants.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed.
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Artificial miRNAs and target-mimics as potential tools for crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Integration of biological and information technologies to enhance plant autoluminescence.The Plant cell · 2024Article
- Multiscale chromatin dynamics and high entropy in plant iPSC ancestors.Journal of cell science · 2024Article
- Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice.Scientific reports · 2021Article
- A Versatile and Efficient Plant Protoplast Platform for Genome Editing by Cas9 RNPs.Frontiers in genome editing · 2021Article
- Protoplast: A Valuable Toolbox to Investigate Plant Stress Perception and Response.Frontiers in plant science · 2021Review
- Silencing AC1 of Tomato leaf curl virus using artificial microRNA confers resistance to leaf curl disease in transgenic tomato.Plant cell reports · 2020Article
- Multiple Xanthomonas campestris pv. campestris 8004 type III effectors inhibit immunity induced by flg22.Planta · 2020Article
- BRAHMA-interacting proteins BRIP1 and BRIP2 are core subunits of Arabidopsis SWI/SNF complexes.Nature plants · 2020Article
- Artificial Small RNA-Based Silencing Tools for Antiviral Resistance in Plants.Plants (Basel, Switzerland) · 2020Review
- Engineered Artificial MicroRNA Precursors Facilitate Cloning and Gene Silencing in Arabidopsis and Rice.International journal of molecular sciences · 2019Article
- Artificial microRNA-mediated resistance to cucumber green mottle mosaic virus in Nicotiana benthamiana.Planta · 2019Article
- Virus-Derived Small Interfering RNAs Affect the Accumulations of Viral and Host Transcripts in Maize.Viruses · 2018Article
- Engineering Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen.Plant physiology · 2018Article
- A potent Cas9-derived gene activator for plant and mammalian cells.Nature plants · 2017Article
- A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants.Plant physiology · 2017Article
- Discovery of Putative Herbicide Resistance Genes and Its Regulatory Network in Chickpea Using Transcriptome Sequencing.Frontiers in plant science · 2017Article
- Polycistronic artificial miRNA-mediated resistance to Wheat dwarf virus in barley is highly efficient at low temperature.Molecular plant pathology · 2016Article
- Artificial microRNA mediated gene silencing in plants: progress and perspectives.Plant molecular biology · 2014Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Artificial miRNA (amiRNA) technology offers highly specific gene silencing in diverse plant species. The principal challenge in amiRNA application is to select potent amiRNAs from hundreds of bioinformatically designed candidates to enable maximal target gene silencing at the protein level. To address this issue, we developed the epitope-tagged protein-based amiRNA (ETPamir) screens, in which single or multiple potential target genes encoding epitope-tagged proteins are constitutively or inducibly coexpressed with individual amiRNA candidates in plant protoplasts. Accumulation of tagged proteins, detected by immunoblotting with commercial tag antibodies, inversely and quantitatively reflects amiRNA efficacy in vivo. The core procedure, from protoplast isolation to identification of optimal amiRNA, can be completed in 2-3 d. The ETPamir screens circumvent the limited availability of plant antibodies and the complexity of plant amiRNA silencing at target mRNA and/or protein levels. The method can be extended to verify predicted target genes for endogenous plant miRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.