ArticleInternational journal of medical microbiology : IJMM2014
Characterization of lactate utilization and its implication on the physiology of Haemophilus influenzae.
Article in International journal of medical microbiology : IJMM, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Gut metabolite L-lactate supportsProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection.Frontiers in cellular and infection microbiology · 2024Article
- Diversification of signal identity and modus operandi of the Haemophilus influenzae PAS-less ArcB sensor kinase.PloS one · 2024Article
- Hemin availability induces coordinated DNA methylation and gene expression changes inmSystems · 2023Article
- Access to highly specialized growth substrates and production of epithelial immunomodulatory metabolites determine survival of Haemophilus influenzae in human airway epithelial cells.PLoS pathogens · 2022Article
- Lactate cross-talk in host-pathogen interactions.The Biochemical journal · 2021Review
- Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae.Cell host & microbe · 2020Article
- Article
- In vivo repressed genes ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria.Nature communications · 2016Article
- No development of ciprofloxacin resistance in the Haemophilus species associated with pneumonia over a 10-year study.BMC infectious diseases · 2015Observational
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Haemophilus influenzae is a Gram-negative bacillus and a frequent commensal of the human nasopharynx. Earlier work demonstrated that in H. influenzae type b, l-lactate metabolism is associated with serum resistance and in vivo survival of the organism. To further gain insight into lactate utilization of the non-typeable (NTHi) isolate 2019 and laboratory prototype strain Rd KW20, deletion mutants of the l-lactate dehydrogenase (lctD) and permease (lctP) were generated and characterized. It is shown, that the apparent KM of l-lactate uptake is 20.1μM as determined for strain Rd KW20. Comparison of the COPD isolate NTHi 2019-R with the corresponding lctP knockout strain for survival in human serum revealed no lactate dependent serum resistance. In contrast, we observed a 4-fold attenuation of the mutant strain in a murine model of nasopharyngeal colonization. Characterization of lctP transcriptional control shows that the lactate utilization system in H. influenzae is not an inductor inducible system. Rather negative feedback regulation was observed in the presence of l-lactate and this is dependent on the ArcAB regulatory system. Additionally, for 2019 it was found that lactate may have signaling function leading to increased cell growth in late log phase under conditions where no l-lactate is metabolized. This effect seems to be ArcA independent and was not observed in strain Rd KW20. We conclude that l-lactate is an important carbon-source and may act as host specific signal substrate which fine tunes the globally acting ArcAB regulon and may additionally affect a yet unknown signaling system and thus may contribute to enhanced in vivo survival.
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Registered trials
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