ArticleStem cell reports2014
Five transcription factors and FGF pathway inhibition efficiently induce erythroid differentiation in the epiblast.
Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- ETV2 induces endothelial, but not hematopoietic, lineage specification in birds.Life science alliance · 2024Article
- NPAS4L is involved in avian hemangioblast specification.Haematologica · 2020Article
- Systematic analysis of transcription start sites in avian development.PLoS biology · 2017Article
- Defining the Minimal Factors Required for Erythropoiesis through Direct Lineage Conversion.Cell reports · 2016Article
- An expandable, inducible hemangioblast state regulated by fibroblast growth factor.Stem cell reports · 2014Article
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Authors and funding
2 authors.
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Abstract
Primitive erythropoiesis follows a stereotypic developmental program of mesoderm ventralization and internalization, hemangioblast formation and migration, and erythroid lineage specification. Induction of erythropoiesis is inefficient in either ES/iPS cells in vitro or nonhemangioblast cell populations in vivo. Using the chick model, we report that epiblast cells can be directly and efficiently differentiated into the erythroid lineage by expressing five hematopoietic transcription regulators (SCL+LMO2+GATA2+LDB1+E2A) and inhibiting the FGF pathway. We show that these five genes are expressed with temporal specificity during normal erythropoiesis. Initiation of SCL and LMO2 expression requires FGF activity, whereas erythroid differentiation is enhanced by FGF inhibition. The lag between hematopoiesis and erythropoiesis is attributed to sequential coregulator expression and hemangioblast migration. Globin gene transcription can be ectopically and prematurely induced by manipulating the availability of these factors and the FGF pathway activity. We propose that similar approaches can be taken for efficient erythroid differentiation in vitro.
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