ArticleTheScientificWorldJournal2014
Optimization and validation of indirect ELISA using truncated TssB protein for the serodiagnosis of glanders amongst equines.
Article in TheScientificWorldJournal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Comparison of immunogenicity of 17 Burkholderia mallei antigens and whole cell lysate using indirect ELISA.The Journal of veterinary medical science · 2025Article
- Development and validation of a chemiluminescent western blot assay for glanders (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Influence of genomic variations on glanders serodiagnostic antigens using integrative genomic and transcriptomic approaches.Frontiers in veterinary science · 2023Article
- A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections.Virulence · 2021Article
- Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae.Frontiers in veterinary science · 2021Article
- Seroprevalence of equine glanders in horses in the central and eastern parts of Mongolia.The Journal of veterinary medical science · 2020Article
- Article
- Recent Advances in Burkholderia mallei and B. pseudomallei Research.Current tropical medicine reports · 2015Article
- Incidence of Burkholderia mallei infection among indigenous equines in India.Veterinary record open · 2015Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo express truncated TssB protein of Burkholderia mallei and to evaluate its diagnostic efficacy for serological detection of glanders among equines. MATERIALS AND
methodsIn an attempt to develop recombinant protein based enzyme-linked immunosorbent assay (ELISA), N-terminal 200 amino acid sequences of B. mallei TssB protein-a type 6 secretory effector protein--were expressed in prokaryotic expression system. Diagnostic potential of recombinant TssB protein was evaluated in indirect ELISA using a panel of glanders positive (n = 49), negative (n = 30), and field serum samples (n = 1811). Cross-reactivity of the assay was assessed with equine disease control serum and human melioidosis positive serum.
resultsIn comparison to CFT, diagnostic sensitivity and specificity of ELISA were 99.7% and 100%, respectively.
conclusionsThe indirect ELISA method using the truncated TssB offered safer and more rapid and efficient means of serodiagnosis of glanders in equines. These data highlight the use of TssB as potential diagnostic antigen for serological diagnosis of glanders.
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