ArticleMolecular and cellular biology1989
Synthesis of p36 and p35 is increased when U-937 cells differentiate in culture but expression is not inducible by glucocorticoids.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 55 citations in OpenAlex.
- Upregulation of annexin A1 expression by butyrate in human colon adenocarcinoma cells: role of p53, NF-Y, and p38 mitogen-activated protein kinase.Molecular and cellular biology · 2008Article
- Evidence for specific annexin I-binding proteins on human monocytes.The Biochemical journal · 1996Article
- Translocation of lipocortin (annexin) 1 to the membrane of U937 cells induced by phorbol ester, but not by dexamethasone.British journal of pharmacology · 1996Article
- Polyamines are essential for cell transformation by pp60v-src: delineation of molecular events relevant for the transformed phenotype.The Journal of cell biology · 1993Article
- Annexin 5 as a potential regulator of annexin 1 phosphorylation by protein kinase C. In vitro inhibition compared with quantitative data on annexin distribution in human endothelial cells.The Biochemical journal · 1993Article
- Distinct cellular expression pattern of annexins in Hydra vulgaris.The Journal of cell biology · 1992Article
- Lipocortin 1 mediates dexamethasone-induced growth arrest of the A549 lung adenocarcinoma cell line.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Elevated expression of annexin II (lipocortin II, p36) in a multidrug resistant small cell lung cancer cell line.British journal of cancer · 1992Article
- Protein-synthesis-dependent induction of annexin I by glucocorticoid.The Biochemical journal · 1991Article
- Annexins: calcium-binding proteins of multi-functional importance?Medical microbiology and immunology · 1991Review
- The crystal and molecular structure of human annexin V, an anticoagulant protein that binds to calcium and membranes.The EMBO journal · 1990Article
- Expression of annexins as a function of cellular growth state.The Journal of cell biology · 1990Article
- Glucocorticoids inhibit prostaglandin synthesis not only at the level of phospholipase A2 but also at the level of cyclo-oxygenase/PGE isomerase.British journal of pharmacology · 1989Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
p36 and p35 are distinct but related proteins that share many structural and biochemical features which were first identified as major substrates for protein-tyrosine kinases. Subsequently, both proteins have been shown to be Ca2+-, phospholipid-, and F-actin-binding proteins that underlie the plasma membrane and are associated with the cortical cytoskeleton. Recent reports have claimed that these proteins function as lipocortins, i.e., phospholipase A2 inhibitors that mediate the anti-inflammatory action of glucocorticoids. To investigate this possibility and to learn more about the functions of p36 and p35, we used human-specific anti-p36 and anti-p35 monoclonal antibodies to determine whether the expression or secretion of either protein was inducible by dexamethasone in the human U-937 myeloid cell line and in other human cell types. Additionally, we examined the levels of mRNA for both proteins. No effect of dexamethasone was observed on p36 or p35 expression at either the mRNA or protein level, nor were these proteins secreted under any of the culture conditions investigated. However, it was observed that in these cells the rate of synthesis and accumulation of both proteins was increased when the U-937 cells were induced to differentiate in culture to adherent macrophagelike cells. This offers a model system with which to study the control of p36 and p35 expression.
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