ArticleJournal of clinical microbiology2014
Massive parallel sequencing provides new perspectives on bacterial brain abscesses.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Article
- Odontogenic Brain Abscess in a Hereditary Haemorrhagic Telangiectasia (HHT) Patient: Case Report with a Comprehensive Literature Review.Tropical medicine and infectious disease · 2026Article
- Distribution of nicotinamide phosphoribosyltransferase in genusFrontiers in microbiology · 2026Article
- Metagenomic Next-Generation Sequencing for Brain Abscess: Improved Pathogen Detection, Targeted Antimicrobial Therapy, and Association with Fewer Surgical Interventions.Infection and drug resistance · 2026Article
- Review
- Oral microbiome and nitric oxide biomarkers in older people with mild cognitive impairment andPNAS nexus · 2025Article
- Oral pathologic conditions and impaired cytokine response in patients with previous cerebral abscess or cervical necrotizing soft tissue infection.Frontiers in cellular and infection microbiology · 2025Observational
- Augmented pathogen detection in brain abscess using metagenomic next-generation sequencing: a retrospective cohort study.Microbiology spectrum · 2024Article
- The utility of 16S rRNA gene sequencing on intraoperative specimens from intracranial infections: an 8-year study in a regional UK neurosurgical unit.British journal of neurosurgery · 2024Article
- Microbiology ofOpen forum infectious diseases · 2024Article
- Polymicrobial brain abscesses: A complex condition with diagnostic and therapeutic challenges.Journal of neuropathology and experimental neurology · 2024Review
- Microbiological diagnosis of pleural infections: a comparative evaluation of a novel syndromic real-time PCR panel.Microbiology spectrum · 2024Article
- Bacterial symbionts in oral niche use type VI secretion nanomachinery for fitness increase against pathobionts.iScience · 2024Article
- Polymicrobial infections with specificJournal of oral microbiology · 2024Review
- Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum: A Distinct Entity of Pleural Infections.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Article
- Cryptococcal meningoencephalitis with Actinomyces odontolyticus sepsis: a case report and literature review.BMC infectious diseases · 2023Review
- Culture on Selective Media and Amplicon-Based Sequencing of 16S rRNA from Spontaneous Brain Abscess-the View from the Diagnostic Laboratory.Microbiology spectrum · 2022Article
- The Brief Case: Streptococcus intermedius Brain Abscesses in an Otherwise Healthy Young Man Diagnosed by 16S rRNA Gene Sequencing.Journal of clinical microbiology · 2022Article
- Review
- SpontaneousClinical case reports · 2022Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid development within the field of massive parallel sequencing (MPS) is about to bring this technology within reach for diagnostic microbiology laboratories. We wanted to explore its potential for improving diagnosis and understanding of polymicrobial infections, using bacterial brain abscesses as an example. We conducted a prospective nationwide study on bacterial brain abscesses. Fifty-two surgical samples were included over a 2-year period. The samples were categorized as either spontaneous intracerebral, spontaneous subdural, or postoperative. Bacterial 16S rRNA genes were amplified directly from the specimens and sequenced using Ion Torrent technology, with an average of 500,000 reads per sample. The results were compared to those from culture- and Sanger sequencing-based diagnostics. Compared to culture, MPS allowed for triple the number of bacterial identifications. Aggregatibacter aphrophilus, Fusobacterium nucleatum, and Streptococcus intermedius or combinations of them were found in all spontaneous polymicrobial abscesses. F. nucleatum was systematically detected in samples with anaerobic flora. The increased detection rate for Actinomyces spp. and facultative Gram-negative rods further revealed several species associations. We suggest that A. aphrophilus, F. nucleatum, and S. intermedius are key pathogens for the establishment of spontaneous polymicrobial brain abscesses. In addition, F. nucleatum seems to be important for the development of anaerobic flora. MPS can accurately describe polymicrobial specimens when a sufficient number of reads is used to compensate for unequal species concentrations and principles are defined to discard contaminant bacterial DNA in the subsequent data analysis. This will contribute to our understanding of how different types of polymicrobial infections develop.
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