ArticleProtein & cell2014
Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence.
Article in Protein & cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- The Conserved GTPase LepA May Contribute to the Final Proper Stabilization of the 3' Domain of the 30S Subunit During Ribosome Assembly.International journal of molecular sciences · 2026Article
- Analysis of stress response in multiple bacterial pathogens using a network biology approach.Scientific reports · 2025Article
- Article
- Purification and Characterization of Authentic 30S Ribosomal Precursors Induced by Heat Shock.International journal of molecular sciences · 2023Article
- GTPase Era at the heart of ribosome assembly.Frontiers in molecular biosciences · 2023Review
- Structural Consequences of Deproteinating the 50S Ribosome.Biomolecules · 2022Article
- Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria.Microorganisms · 2022Review
- Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast.RNA (New York, N.Y.) · 2022Article
- RbfA Is Involved in Two Important Stages of 30S Subunit Assembly: Formation of the Central Pseudoknot and Docking of Helix 44 to the Decoding Center.International journal of molecular sciences · 2021Article
- Ribosome assembly defects subvert initiation Factor3 mediated scrutiny of bona fide start signal.Nucleic acids research · 2019Article
- Role of Era in assembly and homeostasis of the ribosomal small subunit.Nucleic acids research · 2019Article
- The RNA-binding protein Hfq is important for ribosome biogenesis and affects translation fidelity.The EMBO journal · 2018Article
- Structure and dynamics of bacterial ribosome biogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- The impact of recent improvements in cryo-electron microscopy technology on the understanding of bacterial ribosome assembly.Nucleic acids research · 2017Review
- Binding properties of YjeQ (RsgA), RbfA, RimM and Era to assembly intermediates of the 30S subunit.Nucleic acids research · 2016Article
- YphC and YsxC GTPases assist the maturation of the central protuberance, GTPase associated region and functional core of the 50S ribosomal subunit.Nucleic acids research · 2016Article
- Mutations of ribosomal protein S5 suppress a defect in late-30S ribosomal subunit biogenesis caused by lack of the RbfA biogenesis factor.RNA (New York, N.Y.) · 2015Article
- The C-terminal helix in the YjeQ zinc-finger domain catalyzes the release of RbfA during 30S ribosome subunit assembly.RNA (New York, N.Y.) · 2015Article
- Modulation of decoding fidelity by ribosomal proteins S4 and S5.Journal of bacteriology · 2015Article
- The large ribosomal subunit protein L9 enables the growth of EF-P deficient cells and enhances small subunit maturation.PloS one · 2015Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The in vivo assembly of ribosomal subunits is a highly complex process, with a tight coordination between protein assembly and rRNA maturation events, such as folding and processing of rRNA precursors, as well as modifications of selected bases. In the cell, a large number of factors are required to ensure the efficiency and fidelity of subunit production. Here we characterize the immature 30S subunits accumulated in a factor-null Escherichia coli strain (∆rsgA∆rbfA). The immature 30S subunits isolated with varying salt concentrations in the buffer system show interesting differences on both protein composition and structure. Specifically, intermediates derived under the two contrasting salt conditions (high and low) likely reflect two distinctive assembly stages, the relatively early and late stages of the 3' domain assembly, respectively. Detailed structural analysis demonstrates a mechanistic coupling between the maturation of the 5' end of the 17S rRNA and the assembly of the 30S head domain, and attributes a unique role of S5 in coordinating these two events. Furthermore, our structural results likely reveal the location of the unprocessed terminal sequences of the 17S rRNA, and suggest that the maturation events of the 17S rRNA could be employed as quality control mechanisms on subunit production and protein translation.
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