ArticleMethods in molecular biology (Clifton, N.J.)2014
Using homogeneous primary neuron cultures to study fundamental aspects of HSV-1 latency and reactivation.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Axonal entry and retrograde transport define HSV-1 latency establishment and reactivation potential in neurons.bioRxiv : the preprint server for biology · 2026Article
- Viral Ubiquitin Ligase Stimulates Selective Host MicroRNA Expression by Targeting ZEB Transcriptional Repressors.Viruses · 2017Article
- Modeling HSV-1 Latency in Human Embryonic Stem Cell-Derived Neurons.Pathogens (Basel, Switzerland) · 2017Article
- Immune Escape via a Transient Gene Expression Program Enables Productive Replication of a Latent Pathogen.Cell reports · 2017Article
- The Characteristics of Herpes Simplex Virus Type 1 Infection in Rhesus Macaques and the Associated Pathological Features.Viruses · 2017Article
- HSV1 MicroRNA Modulation of GPI Anchoring and Downstream Immune Evasion.Cell reports · 2016Article
- Herpes Simplex Virus 1 Infection of Tree Shrews Differs from That of Mice in the Severity of Acute Infection and Viral Transcription in the Peripheral Nervous System.Journal of virology · 2016Article
- An in vitro model of latency and reactivation of varicella zoster virus in human stem cell-derived neurons.PLoS pathogens · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We describe a primary neuronal culture system suitable for molecular characterization of herpes simplex virus type 1 (HSV-1) infection, latency, and reactivation. While several alternative models are available, including infections of live animal and explanted ganglia, these are complicated by the presence of multiple cell types, including immune cells, and difficulties in manipulating the neuronal environment. The highly pure neuron culture system described here can be readily manipulated and is ideal for molecular studies that focus exclusively on the relationship between the virus and host neuron, the fundamental unit of latency. As such it allows for detailed investigations of both viral and neuronal factors involved in the establishment and maintenance of HSV-1 latency and in viral reactivation induced by defined stimuli.
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Registered trials
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