ArticleHistochemistry and cell biology2014
A fast, low cost, and highly efficient fluorescent DNA labeling method using methyl green.
Article in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- Optical Tissue Clearing and Small-Molecule Labeling of Paraffin-Embedded Breast Cancer and Axillary Lymph Node Human Tissue Samples.Bio-protocol · 2026Article
- Modulation of Methyl Green's Optical Properties by Solvent Polarity, Encapsulation, and Dynamic Magnetic Fields.Journal of fluorescence · 2026Article
- Fluorescence dye-conjugated magnetic core-shell silica nanoparticles for enhanced nucleic acid visualization.RSC advances · 2025Article
- Affinity-Based Protein Profiling Reveals IDH2 as a Mitochondrial Target of Cannabinol in Receptor-Independent Neuroprotection.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Visualizing Intracellular Localization of Natural-Product-Based Chemical Probes Using Click-Correlative Light and Electron Microscopy.ACS chemical biology · 2025Article
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- Boron Clusters Alter the Membrane Permeability of Dicationic Fluorescent DNA-Staining Dyes.ACS omega · 2023Article
- Stem cell proliferation and differentiation during larval metamorphosis of the model tapewormFrontiers in cellular and infection microbiology · 2023Article
- Synthesis, Conformational Analysis and ctDNA Binding Studies of Flavonoid Analogues Possessing the 3,5-di-Antioxidants (Basel, Switzerland) · 2022Article
- Zebrafish Slit2 and Slit3 Act Together to Regulate Retinal Axon Crossing at the Midline.Journal of developmental biology · 2022Article
- A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules Against the Intracellular Form of Leishmania infantum.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Click-correlative light and electron microscopy (click-AT-CLEM) for imaging and tracking azido-functionalized sphingolipids in bacteria.Scientific reports · 2021Article
- The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove.Current research in toxicology · 2021Article
- Cellular maps of gastrointestinal organs: getting the most from tissue clearing.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- Scalable Labeling for Cytoarchitectonic Characterization of Large Optically Cleared Human Neocortex Samples.Scientific reports · 2019Article
- Divergent Axin and GSK-3 paralogs in the beta-catenin destruction complexes of tapeworms.Development genes and evolution · 2019Article
- Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.Frontiers in neuroanatomy · 2019Article
- Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasing need for multiple-labeling of cells and whole organisms for fluorescence microscopy has led to the development of hundreds of fluorophores that either directly recognize target molecules or organelles, or are attached to antibodies or other molecular probes. DNA labeling is essential to study nuclear-chromosomal structure, as well as for gel staining, but also as a usual counterstain in immunofluorescence, FISH or cytometry. However, there are currently few reliable red to far-red-emitting DNA stains that can be used. We describe herein an extremely simple, inexpensive and robust method for DNA labeling of cells and electrophoretic gels using the very well-known histological stain methyl green (MG). MG used in very low concentrations at physiological pH proved to have relatively narrow excitation and emission spectra, with peaks at 633 and 677 nm, respectively, and a very high resistance to photobleaching. It can be used in combination with other common DNA stains or antibodies without any visible interference or bleed-through. In electrophoretic gels, MG also labeled DNA in a similar way to ethidium bromide, but, as expected, it did not label RNA. Moreover, we show here that MG fluorescence can be used as a stain for direct measuring of viability by both microscopy and flow cytometry, with full correlation to ethidium bromide staining. MG is thus a very convenient alternative to currently used red-emitting DNA stains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.