ArticlePloS one2014
Gene and protein expression in response to different growth temperatures and oxygen availability in Burkholderia thailandensis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
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- Differentiation in pyroptosis induction by Burkholderia pseudomallei and Burkholderia thailandensis in primary human monocytes, a possible cause of sepsis in acute melioidosis patients.PLoS neglected tropical diseases · 2024Article
- 'A plant's major strength in rhizosphere': the plant growth promoting rhizobacteria.Archives of microbiology · 2023Review
- Methods to Evaluate Bacterial Motility and Its Role in Bacterial-Host Interactions.Microorganisms · 2022Review
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- Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages.BMC microbiology · 2019Article
- Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3.Scientific reports · 2019Article
- Mutations causing low level antibiotic resistance ensure bacterial survival in antibiotic-treated hosts.Scientific reports · 2018Article
- Effect of temperature on Burkholderia pseudomallei growth, proteomic changes, motility and resistance to stress environments.Scientific reports · 2018Article
- Quantitative RNA-seq Analysis Unveils Osmotic and Thermal Adaptation Mechanisms Relevant for Ectoine Production inFrontiers in microbiology · 2018Article
- The Small RNA ErsA ofFrontiers in microbiology · 2018Article
- WoPPER: Web server for Position Related data analysis of gene Expression in Prokaryotes.Nucleic acids research · 2017Article
- Genomic and transcriptomic comparison between Staphylococcus aureus strains associated with high and low within herd prevalence of intra-mammary infection.BMC microbiology · 2017Article
- In-Frame and Unmarked Gene Deletions in Burkholderia cenocepacia via an Allelic Exchange System Compatible with Gateway Technology.Applied and environmental microbiology · 2015Article
- A Genomic, Transcriptomic and Proteomic Look at the GE2270 Producer Planobispora rosea, an Uncommon Actinomycete.PloS one · 2015Article
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia thailandensis, although normally avirulent for mammals, can infect macrophages in vitro and has occasionally been reported to cause pneumonia in humans. It is therefore used as a model organism for the human pathogen B. pseudomallei, to which it is closely related phylogenetically. We characterized the B. thailandensis clinical isolate CDC2721121 (BtCDC272) at the genome level and studied its response to environmental cues associated with human host colonization, namely, temperature and oxygen limitation. Effects of the different growth conditions on BtCDC272 were studied through whole genome transcription studies and analysis of proteins associated with the bacterial cell surface. We found that growth at 37°C, compared to 28°C, negatively affected cell motility and flagella production through a mechanism involving regulation of the flagellin-encoding fliC gene at the mRNA stability level. Growth in oxygen-limiting conditions, in contrast, stimulated various processes linked to virulence, such as lipopolysaccharide production and expression of genes encoding protein secretion systems. Consistent with these observations, BtCDC272 grown in oxygen limitation was more resistant to phagocytosis and strongly induced the production of inflammatory cytokines from murine macrophages. Our results suggest that, while temperature sensing is important for regulation of B. thailandensis cell motility, oxygen limitation has a deeper impact on its physiology and constitutes a crucial environmental signal for the production of virulence factors.
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