Evidence map›Paper›PMID 24671093›Full record

ArticlePloS one2014

Probenecid blocks human P2X7 receptor-induced dye uptake via a pannexin-1 independent mechanism.

Archana Bhaskaracharya, Phuong Dao-Ung, Iman Jalilian, Mari Spildrejorde, Kristen K Skarratt, Stephen J Fuller, Ronald Sluyter, Leanne Stokes

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
8.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 77 citations in OpenAlex.

  1. Article
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  6. The MAPK Response to Virus Infection Is Modified by Probenecid.Current issues in molecular biology · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Archana BhaskaracharyaSydney Medical School Nepean, University of Sydney, Nepean Hospital, Penrith, New South Wales, Australia.
Phuong Dao-UngSydney Medical School Nepean, University of Sydney, Nepean Hospital, Penrith, New South Wales, Australia.
Iman JalilianSchool of Biological Sciences, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, Australia.
Mari SpildrejordeSchool of Biological Sciences, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, Australia.
Kristen K SkarrattSydney Medical School Nepean, University of Sydney, Nepean Hospital, Penrith, New South Wales, Australia.
Stephen J FullerSydney Medical School Nepean, University of Sydney, Nepean Hospital, Penrith, New South Wales, Australia.
Ronald SluyterSchool of Biological Sciences, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, Australia.
Leanne StokesSydney Medical School Nepean, University of Sydney, Nepean Hospital, Penrith, New South Wales, Australia; Health Innovations Research Institute, School of Medical Sciences, RMIT University, Bundoora, Melbourne, Victoria, Australia.
Nepean Hospital · AUUniversity of Sydney · AUUniversity of Wollongong · AUIllawarra Health and Medical Research Institute · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

P2X7 is a ligand-gated ion channel which is activated by ATP and displays secondary permeability characteristics. The mechanism of development of the secondary permeability pathway is currently unclear, although a role for the hemichannel protein pannexin-1 has been suggested. In this study we investigated the role of pannexin-1 in P2X7-induced dye uptake and ATP-induced IL-1β secretion from human monocytes. We found no pharmacological evidence for involvement of pannexin-1 in P2X7-mediated dye uptake in transfected HEK-293 cells with no inhibition seen for carbenoxolone and the pannexin-1 mimetic inhibitory peptide, 10Panx1. However, we found that probenecid inhibited P2X7-induced cationic and anionic dye uptake in stably transfected human P2X7 HEK-293 cells. An IC50 value of 203 μM was calculated for blockade of ATP-induced responses at human P2X7. Probenecid also reduced dye uptake and IL-1β secretion from human CD14+ monocytes whereas carbenoxolone and 10Panx1 showed no inhibitory effect. Patch clamp and calcium indicator experiments revealed that probenecid directly blocks the human P2X7 receptor.

Indexed as

Adenosine TriphosphateBiological Transport, ActiveCalcium SignalingConnexinsEthidiumFluorescent DyesHEK293 CellsHumansInhibitory Concentration 50Interleukin-1betaIsoquinolinesLipopolysaccharidesMonocytesNerve Tissue ProteinsProbenecidPurinergic P2X Receptor AntagonistsAdenosine TriphosphateConnexinsEthidiumFluorescent DyesInterleukin-1betaIsoquinolinesLipopolysaccharideslucifer yellowNerve Tissue ProteinsPANX1 protein, humanProbenecidPurinergic P2X Receptor AntagonistsReceptors, Purinergic P2X7

Identifiers

PMID24671093
PMCPMC3966854
OpenAlexW2040278093

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.