ArticleScientific reports2014
CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Application of Gene Editing Technology in Poultry.Veterinary sciences · 2026Review
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants.Plants (Basel, Switzerland) · 2026Review
- CRISPR-Mediated Silkworm: The Oncoming Agricultural Revolutions and a Rising Model Organism.Genes · 2026Review
- Integrated Genomic Analysis Reveals the Impact of Avermectin on Chromatin Structure and Gene Expression Regulation inInsects · 2025Article
- Establishment of CRISPR/Cas9-based knock-in in a hemimetabolous insect: targeted gene tagging in the cricket Gryllus bimaculatus.Development (Cambridge, England) · 2025Article
- High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism,Genome research · 2024Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- CRISPR-based gene editing of non-homologous end joining factors biases DNA repair pathway choice toward single-strand annealing inCurrent research in biotechnology · 2023Article
- Article
- KPI5 Is Involved in the Regulation of the Expression of Antibacterial Peptide Genes and Hemolymph Melanization in the Silkworm,Frontiers in immunology · 2022Article
- Advances in Editing Silkworms (Insects · 2021Review
- Article
- CRISPR-Mediated Endogenous Activation of Fibroin Heavy Chain Gene Triggers Cellular Stress Responses inInsects · 2021Article
- The Ku complex: recent advances and emerging roles outside of non-homologous end-joining.Cellular and molecular life sciences : CMLS · 2021Review
- Toward precise CRISPR DNA fragment editing and predictable 3D genome engineering.Journal of molecular cell biology · 2021Review
- Expansion of the CRISPR/Cas Genome-Sculpting Toolbox: Innovations, Applications and Challenges.Molecular diagnosis & therapy · 2021Review
- Genetic Manipulation of Ticks: A Paradigm Shift in Tick and Tick-Borne Diseases Research.Frontiers in cellular and infection microbiology · 2021Review
- An Efficient Workflow for Screening and Stabilizing CRISPR/Cas9-Mediated Mutant Lines inMethods and protocols · 2020Article
- Various Aspects of a Gene Editing System-CRISPR-Cas9.International journal of molecular sciences · 2020Review
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Authors and funding
10 authors.
Funding
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Abstract
CRISPR/Cas9, a bacterial adaptive immune system derived genome-editing technique, has become to be one of the most compelling topics in biotechnology. Bombyx mori is an economically important insect and a model organism for studying lepidopteran and arthropod biology. Here we reported highly efficient and multiplex genome editing in B. mori cell line and heritable site-directed mutagenesis of Bmku70, which is required for NHEJ pathway and also related to antigen diversity, telomere length maintenance and subtelomeric gene silencing, using CRISPR/Cas9 system. We established a simple and practicable method and obtained several Bmku70 knockout B. mori lines, and showed that the frequency of HR was increased in embryos of the Bmku70 knockout B. mori. The mutant lines obtained in this study could be a candidate genetic resource for efficient knock-in and fundamental research of DNA repair in B. mori. We also provided a strategy and procedure to perform heritable genome editing of target genes with no significant phenotype effect.
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