ArticleScientific reports2014
Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 85 citations in OpenAlex.
- Pancreatic organoids and organoid-on-a-chip platforms: from disease modeling to precision therapy.Journal of translational medicine · 2026Review
- Quality control strategy for differentiating induced pluripotent stem cells into pancreatic β-cells before transplantation.Regenerative medicine · 2026Review
- Islets in the body are never flat: transitioning from two-dimensional (2D) monolayer culture to three-dimensional (3D) spheroid for better efficiency in the generation of functional hPSC-derived pancreatic β cells in vitro.Cell communication and signaling : CCS · 2023Review
- Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell Properties.Cells · 2023Article
- Stem cells differentiation into insulin-producing cells (IPCs): recent advances and current challenges.Stem cell research & therapy · 2022Review
- Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold.Cell regeneration (London, England) · 2021Article
- Article
- Induced Pluripotent Stem Cells on a Chip: A Self-Contained, Accessible, Pipette-less iPSC Culturing and Differentiation Kit.SLAS technology · 2021Article
- Enhanced differentiation of human pluripotent stem cells into pancreatic endocrine cells in 3D culture by inhibition of focal adhesion kinase.Stem cell research & therapy · 2020Article
- Review
- Signaling Molecules Regulating Pancreatic Endocrine Development from Pluripotent Stem Cell Differentiation.International journal of molecular sciences · 2020Review
- Small Molecule-Induced Pancreatic β-Like Cell Development: Mechanistic Approaches and Available Strategies.International journal of molecular sciences · 2020Review
- Placenta mesenchymal stem cells differentiation toward neuronal-like cells on nanofibrous scaffold.BioImpacts : BI · 2020Article
- Induction of functional islet-like cells from human iPS cells by suspension culture.Regenerative therapy · 2019Article
- A simple method for the generation of insulin producing cells from bone marrow mesenchymal stem cells.In vitro cellular & developmental biology. Animal · 2019Article
- Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture.Scientific reports · 2019Article
- Glucocorticoid Signaling Enhances Expression of Glucose-Sensing Molecules in Immature Pancreatic Beta-Like Cells Derived from Murine Embryonic Stem Cells In Vitro.Stem cells and development · 2018Article
- Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors.Stem cell research & therapy · 2018Article
- Epigenetic Control of Endocrine Pancreas DifferentiationFrontiers in cell and developmental biology · 2018Review
- Defined three-dimensional culture conditions mediate efficient induction of definitive endoderm lineage from human umbilical cord Wharton's jelly mesenchymal stem cells.Stem cell research & therapy · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulin-producing cells (IPCs) derived from human pluripotent stem cells (hPSCs) may be useful in cell therapy and drug discovery for diabetes. Here, we examined various growth factors and small molecules including those previously reported to develop a robust differentiation method for induction of mature IPCs from hPSCs. We established a protocol that induced PDX1-positive pancreatic progenitor cells at high efficiency, and further induced mature IPCs by treatment with forskolin, dexamethasone, Alk5 inhibitor II and nicotinamide in 3D culture. The cells that differentiated into INSULIN-positive and C-PEPTIDE-positive cells secreted insulin in response to glucose stimulation, indicating a functional IPC phenotype. We also found that this method was applicable to different types of hPSCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.