ArticlePloS one2014
AspC-mediated aspartate metabolism coordinates the Escherichia coli cell cycle.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
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- Aspartate aminotransferase is required forbioRxiv : the preprint server for biology · 2026Article
- Molecular interplay between peptidoglycan integrity and outer membrane asymmetry in maintaining cell envelope homeostasis.Journal of bacteriology · 2025Article
- Characterizing Common Factors Affecting Replication Initiation During HInternational journal of molecular sciences · 2025Article
- Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition.Journal of biological engineering · 2025Article
- Regulation of l- and d-Aspartate Transport and Metabolism in Acinetobacter baylyi ADP1.Applied and environmental microbiology · 2022Article
- Expression regulation of multiple key genes to improve L-threonine in Escherichia coli.Microbial cell factories · 2020Article
- Biosynthesis of Amino Acids inMicroorganisms · 2019Article
- Silver-coated magnetic nanocomposites induce growth inhibition and protein changes in foodborne bacteria.Scientific reports · 2019Article
- Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1-1 plasmid.BMC microbiology · 2018Article
- Absence of RstA results in delayed initiation of DNA replication in Escherichia coli.PloS one · 2018Article
- Metabolism Shapes the Cell.Journal of bacteriology · 2017Review
- Article
- The bacterial alarmone (p)ppGpp is required for virulence and controls cell size and survival of Pseudomonas syringae on plants.Environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe fast-growing bacterial cell cycle consists of at least two independent cycles of chromosome replication and cell division. To ensure proper cell cycles and viability, chromosome replication and cell division must be coordinated. It has been suggested that metabolism could affect the Escherichia coli cell cycle, but the idea is still lacking solid evidences. METHODOLOGY/PRINCIPLE
findingsWe found that absence of AspC, an aminotransferase that catalyzes synthesis of aspartate, led to generation of small cells with less origins and slow growth. In contrast, excess AspC was found to exert the opposite effect. Further analysis showed that AspC-mediated aspartate metabolism had a specific effect in the cell cycle, as only extra aspartate of the 20 amino acids triggered production of bigger cells with more origins per cell and faster growth. The amount of DnaA protein per cell was found to be changed in response to the availability of AspC. Depletion of (p)ppGpp by ΔrelAΔspoT led to a slight delay in initiation of replication, but did not change the replication pattern found in the ΔaspC mutant. CONCLUSION/SIGNIFICANCES: The results suggest that AspC-mediated metabolism of aspartate coordinates the E. coli cell cycle through altering the amount of the initiator protein DnaA per cell and the division signal UDP-glucose. Furthermore, AspC sequence conservation suggests similar functions in other organisms.
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