ArticlePloS one2014
Short-chain flavor ester synthesis in organic media by an E. coli whole-cell biocatalyst expressing a newly characterized heterologous lipase.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 66 citations in OpenAlex.
- A chemical language model for molecular taste prediction.NPJ science of food · 2025Article
- Use of genomics & proteomics in studying lipase producing microorganisms & its application.Food chemistry. Molecular sciences · 2024Article
- Microbial production of butyl butyrate: from single strain to cognate consortium.Bioresources and bioprocessing · 2021Review
- Marine Bacterial Esterases: Emerging Biocatalysts for Industrial Applications.Applied biochemistry and biotechnology · 2021Review
- Microbial lipases and their industrial applications: a comprehensive review.Microbial cell factories · 2020Review
- Production of isopropyl and butyl esters by Clostridium mono-culture and co-culture.Journal of industrial microbiology & biotechnology · 2020Article
- Synthesis of flavor esters by a novel lipase from3 Biotech · 2019Article
- Effect of the Immobilization Strategy on the Efficiency and Recyclability of the Versatile Lipase fromMolecules (Basel, Switzerland) · 2019Article
- Sol-Gel Immobilisation of Lipases: Towards Active and Stable Biocatalysts for the Esterification of Valeric Acid.Molecules (Basel, Switzerland) · 2018Article
- Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Enhancing the stability of a carboxylesterase by entrapment in chitosan coated alginate beads.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
- Ultrasound-Assisted Esterification of Valeric Acid to Alkyl Valerates Promoted by Biosilicified Lipases.Frontiers in chemistry · 2018Article
- Transesterification Synthesis of Chloramphenicol Esters with the Lipase from Bacillus amyloliquefaciens.Molecules (Basel, Switzerland) · 2017Article
- Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications.International journal of molecular sciences · 2015Review
- Immobilization and characterization of a new regioselective and enantioselective lipase obtained from a metagenomic library.PloS one · 2015Article
- Article
- Est16, a New Esterase Isolated from a Metagenomic Library of a Microbial Consortium Specializing in Diesel Oil Degradation.PloS one · 2015Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Short-chain aliphatic esters are small volatile molecules that produce fruity and pleasant aromas and flavors. Most of these esters are artificially produced or extracted from natural sources at high cost. It is, however, possible to 'naturally' produce these molecules using biocatalysts such as lipases and esterases. A gene coding for a newly uncovered lipase was isolated from a previous metagenomic study and cloned into E. coli BL21 (DE3) for overexpression using the pET16b plasmid. Using this recombinant strain as a whole-cell biocatalyst, short chain esters were efficiently synthesized by transesterification and esterification reactions in organic media. The recombinant lipase (LipIAF5-2) showed good affinity toward glyceryl trioctanoate and the highest conversion yields were obtained for the transesterification of glyceryl triacetate with methanol. Using a simple cetyl-trimethylammonium bromide pretreatment increased the synthetic activity by a six-fold factor and the whole-cell biocatalyst showed the highest activity at 40°C with a relatively high water content of 10% (w/w). The whole-cell biocatalyst showed excellent tolerance to alcohol and short-chain fatty acid denaturation. Substrate affinity was equally effective with all primary alcohols tested as acyl acceptors, with a slight preference for methanol. The best transesterification conversion of 50 mmol glyceryl triacetate into isoamyl acetate (banana fragrance) provided near 100% yield after 24 hours using 10% biocatalyst loading (w/w) in a fluidized bed reactor, allowing recycling of the biocatalyst up to five times. These results show promising potential for an industrial approach aimed at the biosynthesis of short-chain esters, namely for natural flavor and fragrance production in micro-aqueous media.
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