ArticleThe Journal of clinical endocrinology and metabolism2014
Clinical utility of simultaneous quantitation of 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D by LC-MS/MS involving derivatization with DMEQ-TAD.
Article in The Journal of clinical endocrinology and metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
102 citing papers in PubMed, 2 syntheses or guidelines pooled it, 244 citations in OpenAlex.
- Impact of dairy supplementation on bone acquisition in children's limbs: a 12-month cluster-randomized controlled trial and meta-analysis.Archives of osteoporosis · 2024Pooled it
- Guideline
- Exercise without Weight Loss Prevents Seasonal Decline in Vitamin D Metabolites: The VitaDEx Randomized Controlled Trial.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Trial
- A phase II dose evaluation pilot feasibility randomized controlled trial of cholecalciferol in critically ill children with vitamin D deficiency (VITdAL-PICU study).BMC pediatrics · 2023Trial
- Formula Milk Supplementation and Bone Acquisition in 4-6 Years Chinese Children: A 12-Month Cluster-Randomized Controlled Trial.Nutrients · 2023Trial
- Inhibit progression of coronary artery calcification with vitamin K in hemodialysis patients (the iPACK-HD study): a randomized, placebo-controlled multi-center, pilot trial.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2023Trial
- Trial
- Vitamin D binding protein rs7041 genotype alters vitamin D metabolism in pregnant women.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Trial
- Placental vitamin D metabolism and its associations with circulating vitamin D metabolites in pregnant women.The American journal of clinical nutrition · 2017Trial
- Trial
- Vitamin D Metabolism Varies among Women in Different Reproductive States Consuming the Same Intakes of Vitamin D and Related Nutrients.The Journal of nutrition · 2016Trial
- Epimeric Vitamin D is Not Associated With Arterial Stiffness or Cardiovascular Outcomes in Patients With Advanced CKD.Kidney medicine · 2026Article
- Relationship between inflammatory indicators and bone mineral density in preschool children.Pediatric research · 2026Article
- A rapid LC-MS/MS method with 2-nitrosopyridine derivatization to determine fat-soluble vitamins, revealing a biphasic bile acids association in clinical cohorts.Analytical and bioanalytical chemistry · 2026Article
- Interlaboratory comparison with a reference measurement procedure (RMP) for determining 24,25-dihydroxyvitamin DAnalytical and bioanalytical chemistry · 2026Article
- C3-Epimer Inclusion Influences Vitamin D Deficiency Classification Near Diagnostic Thresholds in Early Childhood.Nutrients · 2026Article
- The Significance of Fibroblast Growth Factor 23 and 24,25-Dihydroxyvitamin D in Dent Disease Type 1.Clinical journal of the American Society of Nephrology : CJASN · 2026Article
- 25-Hydroxyvitamin DCommunications medicine · 2026Article
- Research on vitamin D metabolic regulation in the pathogenesis of related diseases.Frontiers in endocrinology · 2026Review
- Vitamin D-induced hypercalcemia and nephrolithiasis in a 58-year-old woman with biochemical findings suggestive of 24-hydroxylase deficiency: a case report.AME case reports · 2026Article
42 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors at 9 institutions in 5 countries.
Funding
Abstract
contextThe discovery of hypercalcemic diseases due to loss-of-function mutations in 25-hydroxyvitamin D-24-hydroxylase has placed a new demand for sensitive and precise assays for 24,25-dihydroxyvitamin D [24,25-(OH)2D].
objectiveWe describe a novel liquid chromatography and tandem mass spectrometry-based method involving derivatization with DMEQ-TAD {4-[2-(6,7-dimethoxy-4-methyl-3,4-dihydroquinoxalinyl)ethyl]-1,2,4-triazoline-3,5-dione} to simultaneously assay multiple vitamin D metabolites including 25-hydroxyvitamin D (25-OH-D) and 24,25-(OH)2D using 100 μL of serum with a 5-minute run time.
designThe assay uses a newly synthesized internal standard d6-24,25-(OH)2D3 enabling the quantitation of 24,25-(OH)2D3 as well as the determination of the ratio of 25-OH-D3 to 24,25-(OH)2D3, a physiologically useful parameter.
settingWe report data on more than 1000 normal and disease samples involving vitamin D deficiency or hypercalcemia in addition to studies involving knockout mouse models.
resultsThe assay showed good correlation with samples from quality assurance schemes for 25-OH-D (25-OH-D2 and 25-OH-D3) determination (-2% to -5% bias) and exhibited low inter- and intraassay coefficients of variation (4%-7%) and lower limits of quantitation of 0.25-0.45 nmol/L. In clinical studies, we found a strong correlation between serum levels of 25-OH-D3 and 24,25-(OH)2D3 (r(2) = 0.80) in subjects over a broad range of 25-OH-D3 values and a marked lack of production of 24,25-(OH)2D3 below 25 nmol/L of 25-OH-D. The ratio of 25-OH-D3 to 24,25-(OH)2D3, which remained less than 25 in vitamin D-sufficient subjects (serum 25-OH-D < 50 nmol/L) but was greatly elevated (80-100) in patients with idiopathic infantile hypercalcemia.
conclusionsThe new method showed good utility in clinical settings involving vitamin D deficiency; supplementation with vitamin D and idiopathic infantile hypercalcemia, as well as in animal models with ablation of selected cytochrome P450-containing enzymes involved in vitamin D metabolism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.