ArticleMultidisciplinary respiratory medicine2014
Effects of vitamin D supplementation on alveolar macrophage gene expression: preliminary results of a randomized, controlled trial.
Article in Multidisciplinary respiratory medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01967628 (Human Lung Responses to Respiratory Pathogens), which is not on this map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Human Lung Responses to Respiratory Pathogens
Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Seasonal Antimicrobial Activity of the Airway: Post-Hoc Analysis of a Randomized Placebo-Controlled Double-Blind Trial.Nutrients · 2020Trial
- Prenatal vitamin D supplementation mitigates inflammation-related alveolar remodeling in neonatal mice.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Vitamin D supplementation and immune-related markers: an update from nutrigenetic and nutrigenomic studies.The British journal of nutrition · 2022Review
- Oral vitamin D supplementation induces transcriptomic changes in rectal mucosa that are linked to anti-tumour effects.BMC medicine · 2021Article
- Gene Co-Expression Network Analysis Identifies Vitamin D-Associated Gene Modules in Adult Normal Rectal Epithelium Following Supplementation.Frontiers in genetics · 2021Article
- Aerosol 1,25-dihydroxyvitamin D3 supplementation: A strategy to boost anti-tumor innate immune activity.PloS one · 2021Article
- Vitamin DEBioMedicine · 2019Article
- Airway surface liquid from smokers promotes bacterial growth and biofilm formation via iron-lactoferrin imbalance.Respiratory research · 2018Article
- Serum Vitamin D Level as a Risk Factor for Female Genital Tuberculosis (FGTB).Journal of clinical and diagnostic research : JCDR · 2017Article
- Effects of Coal Fly Ash Particulate Matter on the Antimicrobial Activity of Airway Surface Liquid.Environmental health perspectives · 2017Article
- Effect of vitamin DBMJ open respiratory research · 2017Article
- Vitamin D Deficiency Does Not Result in a Breach of Host Defense in Murine Models of Pneumonia.Infection and immunity · 2016Article
- Interferon-γ Inhibits Ebola Virus Infection.PLoS pathogens · 2015Article
- Respiratory infections: Role of Vitamin D and surfactant proteins A and D.Lung India : official organ of Indian Chest SocietyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundVitamin D deficiency has been implicated as a factor in a number of infectious and inflammatory lung diseases. In the lung, alveolar macrophages play a key role in inflammation and defense of infection, but there are little data exploring the immunomodulatory effects of vitamin D on innate lung immunity in humans. The objective of this study was to determine the effects of vitamin D supplementation on gene expression of alveolar macrophages.
methodsWe performed a parallel, double-blind, placebo-controlled, randomized trial to determine the effects of vitamin D on alveolar macrophage gene expression. Vitamin D3 (1000 international units/day) or placebo was administered to adults for three months. Bronchoscopy was performed pre- and post-intervention to obtain alveolar macrophages. Messenger RNA was isolated from the macrophages and subjected to whole genome exon array analysis. The primary outcome was differential gene expression of the alveolar macrophage in response to vitamin D supplementation. Specific genes underwent validation by polymerase chain reaction methods.
resultsFifty-eight subjects were randomized to vitamin D (n = 28) or placebo (n = 30). There was a marginal overall difference between treatment group and placebo group in the change of 25-hydroxyvitaminD levels (4.43 ng/ml vs. 0.2 ng/ml, p = 0.10). Whole genome exon array analysis revealed differential gene expression associated with change in serum vitamin D levels in the treated group. CCL8/MCP-2 was the top-regulated cytokine gene and was further validated.
conclusionsAlthough only a non-significant increased trend was seen in serum vitamin D levels, subjects treated with vitamin D supplementation had immune-related differential gene expression in alveolar macrophages.
trial registrationClinicalTrials.org: NCT01967628.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.