ArticleBiotechnology for biofuels2014
Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain.
Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 80 citations in OpenAlex.
- Systematic Modeling and Identifiability Analysis of 2,3-Butanediol Biosynthesis by Metabolically EngineeredACS omega · 2026Article
- EngineeringBiodesign research · 2025Article
- Klebsiella pneumoniae under xylose pressure: the growth adaptation, antimicrobial susceptibility, global proteomics analysis and role of XylA and XylB proteins.BMC microbiology · 2025Article
- Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production.Applied microbiology and biotechnology · 2024Article
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Metabolic engineering of Corynebacterium glutamicum for efficient production of optically pure (2R,3R)-2,3-butanediol.Microbial cell factories · 2022Article
- Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in L-valine production.World journal of microbiology & biotechnology · 2022Article
- Bioengineering for the industrial production of 2,3-butanediol by the yeast, Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2022Review
- Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis.Microbial cell factories · 2021Article
- A prediction model using 2-propanol and 2-butanone in urine distinguishes breast cancer.Scientific reports · 2021Article
- C4 Bacterial Volatiles Improve Plant Health.Pathogens (Basel, Switzerland) · 2021Review
- Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae.Biotechnology for biofuels · 2021Article
- Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13.Biotechnology for biofuels · 2021Article
- The current strategies and parameters for the enhanced microbial production of 2,3-butanediol.Biotechnology reports (Amsterdam, Netherlands) · 2020Article
- Microbial production of 2,3-butanediol for industrial applications.Journal of industrial microbiology & biotechnology · 2019Review
- Three Capsular Polysaccharide Synthesis-Related Glucosyltransferases, GT-1, GT-2 and WcaJ, Are Associated With Virulence and Phage Sensitivity ofFrontiers in microbiology · 2019Article
- Engineering Microbial Consortia for High-Performance Cellulosic Hydrolyzates-Fed Microbial Fuel Cells.Frontiers in microbiology · 2019Article
- A Smooth-Type, Phage-Resistant Klebsiella pneumoniae Mutant Strain Reveals that OmpC Is Indispensable for Infection by Phage GH-K3.Applied and environmental microbiology · 2018Article
- Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans.Microbial cell factories · 2018Article
- Improved xylose tolerance and 2,3-butanediol production ofBiotechnology for biofuels · 2018Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background2,3-Butanediol (2,3-BD) is a high-value chemical usually produced petrochemically but which can also be synthesized by some bacteria. To date, Klebsiella pneumoniae is the most powerful 2,3-BD producer which can utilize a wide range of substrates. However, many by-products are also produced by K. pneumoniae, such as ethanol, lactate, and acetate, which negatively regulate the 2,3-BD yield and increase the costs of downstream separation and purification.
resultsIn this study, we constructed K. pneumoniae mutants with lactate dehydrogenase (LDH), acetaldehyde dehydrogenase (ADH), and phosphotransacetylase (PTA) deletion individually by suicide vector conjugation.These mutants showed different behavior of production formation. Knock out of ldhA had little influence on the yield of 2,3-BD, whereas knock out of adhE or pta significantly improved the formation of 2,3-BD. The accumulation of the intermediate of 2,3-BD biosynthesis, acetoin, was decreased in all the mutants. The mutants were then tested in five different carbon sources and increased 2,3-BD was observed. Also a double mutant strain with deletion of adhE and ldhA was constructed which resulted in accelerated fermentation and higher 2,3-BD production. In fed-batch culture this strain achieved more than 100 g/L 2,3-BD from glucose with a relatively high yield of 0.49 g/g.
conclusion2,3-BD production was dramatically improved with the inactivation of adhE and pta. The inactivation of ldhA could advance faster cell growth and shorter fermentation time. The double mutant strain with deletion of adhE and ldhA resulted in accelerated fermentation and higher 2,3-BD production. These results provide new insights for industrial production of 2,3-BD by K. pneumoniae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.