ArticleThe Journal of cell biology1989
Expression of mutant keratin cDNAs in epithelial cells reveals possible mechanisms for initiation and assembly of intermediate filaments.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 205 citations in OpenAlex.
- Discovery of keratin function and role in genetic diseases: the year that 1991 was.Molecular biology of the cell · 2016Review
- Microtubule-dependent transport and dynamics of vimentin intermediate filaments.Molecular biology of the cell · 2015Article
- Defining keratin protein function in skin epithelia: epidermolysis bullosa simplex and its aftermath.The Journal of investigative dermatology · 2012Review
- Inroads into the structure and function of intermediate filament networks.Journal of structural biology · 2012Review
- Dynamic relationship of focal contacts and hemidesmosome protein complexes in live cells.The Journal of investigative dermatology · 2010Article
- Introducing intermediate filaments: from discovery to disease.The Journal of clinical investigation · 2009Review
- Epidermolysis bullosa simplex: a paradigm for disorders of tissue fragility.The Journal of clinical investigation · 2009Review
- Intermediate filaments: primary determinants of cell architecture and plasticity.The Journal of clinical investigation · 2009Review
- Insights into the mechanical properties of epithelial cells: the effects of shear stress on the assembly and remodeling of keratin intermediate filaments.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Disorders of keratinisation: from rare to common genetic diseases of skin and other epithelial tissues.The Ulster medical journal · 2007Review
- Motile properties of vimentin intermediate filament networks in living cells.The Journal of cell biology · 1998Article
- Lessons from keratin 18 knockout mice: formation of novel keratin filaments, secondary loss of keratin 7 and accumulation of liver-specific keratin 8-positive aggregates.The Journal of cell biology · 1998Article
- Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis.The Journal of cell biology · 1997Article
- Keith R. Porter Lecture, 1996. Of mice and men: genetic disorders of the cytoskeleton.Molecular biology of the cell · 1997Review
- The function of intermediate filaments in cell shape and cytoskeletal integrity.The Journal of cell biology · 1996Article
- Gene targeting at the mouse cytokeratin 10 locus: severe skin fragility and changes of cytokeratin expression in the epidermis.The Journal of cell biology · 1996Article
- Epidermolysis bullosa simplex: a keratin 5 mutation is a fully dominant allele in epidermal cytoskeleton function.American journal of human genetics · 1995Article
- Expression of an epidermal keratin protein in liver of transgenic mice causes structural and functional abnormalities.The Journal of cell biology · 1995Article
- Keratin 14 gene point mutation in the Köbner and Dowling-Meara types of epidermolysis bullosa simplex as detected by the PASA method.Archives of dermatological research · 1995Article
- Determinants for intracellular sorting of cytoplasmic and nuclear intermediate filaments.The Journal of cell biology · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have deleted cDNA sequences encoding portions of the amino- and carboxy-terminal end of a human type I epidermal keratin K14, and examined the molecular consequences of forcing the expression of these mutants in simple epithelial and squamous cell carcinoma lines. To follow the expression of our mutant products in transfected cells, we have tagged the 3' end of the K14 coding sequence with a sequence encoding an antigenic domain of the neuropeptide substance P. Using DNA transfection and immunohistochemistry (with an antibody against substance P), we have defined the limits of K14 sequence necessary to incorporate into a keratin filament network in vivo without disrupting its architecture. We have also uncovered major differences in the behavior of carboxy- and amino-terminal alpha-helical mutants which do perturb the cytoskeletal network of IFs: whereas carboxy terminal mutants give rise to aggregates of keratin in the cytoplasm, amino-terminal mutants tend to produce aggregates of keratins which seem to localize at the nuclear surface. An examination of the phenotypes generated by the carboxy and amino-terminal mutants and the behavior of cells at late times after transfection suggests a model whereby initiation of filament assembly occurs at discrete sites on the nuclear envelope and filaments grow from the nucleus toward the cytoplasm.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.