ReviewThe Analyst2014
Microfluidic approaches for epithelial cell layer culture and characterisation.
Review in The Analyst, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Contactless Single Cell Extraction From Monolayer Cell Culture Using a MEMS Acoustic Droplet Ejector.IEEE transactions on bio-medical engineering · 2025Article
- Nanoliposomes Permeability in a Microfluidic Drug Delivery Platform across a 3D Hydrogel.Pharmaceutics · 2024Article
- A Microphysiological System for Studying Barrier Health of Live Tissues in Real Time.Research square · 2024Article
- A comprehensive overview of advanced dynamicTissue barriers · 2024Review
- Article
- Stem cells as a therapeutic avenue for active and long-term complications of Necrotizing Enterocolitis.Seminars in pediatric surgery · 2023Article
- Review
- Online Measurement System for Dynamic Flow Bioreactors to Study Barrier Integrity of hiPSC-Based Blood-Brain Barrier In Vitro Models.Bioengineering (Basel, Switzerland) · 2022Article
- Biological Influence of Pulmonary Disease Conditions Induced by Particulate Matter on Microfluidic Lung Chips.Biochip journal · 2022Article
- Development of a hollow fibre-based renal module for active transport studies.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2021Article
- Progress and Challenges in the Use of a Liver-on-a-Chip for Hepatotropic Infectious Diseases.Micromachines · 2021Review
- Bacteriophage uptake by mammalian cell layers represents a potential sink that may impact phage therapy.iScience · 2021Article
- A bioengineering perspective on modelling the intestinal epithelial physiology in vitro.Nature communications · 2020Review
- A microfluidic device forBiomicrofluidics · 2019Article
- A microfluidic biochip for locally confined stimulation of cells within an epithelial monolayer.RSC advances · 2018Article
- Mimicking Epithelial Tissues in Three-Dimensional Cell Culture Models.Frontiers in bioengineering and biotechnology · 2018Review
- Organs-on-chips with integrated electrodes for trans-epithelial electrical resistance (TEER) measurements of human epithelial barrier function.Lab on a chip · 2017Article
- An integrative microfluidically supported in vitro model of an endothelial barrier combined with cortical spheroids simulates effects of neuroinflammation in neocortex development.Biomicrofluidics · 2016Article
- Temporal Monitoring of Differentiated Human Airway Epithelial Cells Using Microfluidics.PloS one · 2015Article
- Structural and functional analysis of endosomal compartments in epithelial cells.Methods in cell biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
In higher eukaryotes, epithelial cell layers line most body cavities and form selective barriers that regulate the exchange of solutes between compartments. In order to fulfil these functions, the cells assume a polarised architecture and maintain two distinct plasma membrane domains, the apical domain facing the lumen and the basolateral domain facing other cells and the extracellular matrix. Microfluidic biochips offer the unique opportunity to establish novel in vitro models of epithelia in which the in vivo microenvironment of epithelial cells is precisely reconstituted. In addition, analytical tools to monitor biologically relevant parameters can be directly integrated on-chip. In this review we summarise recently developed biochip designs for culturing epithelial cell layers. Since endothelial cell layers, which line blood vessels, have similar barrier functions and polar organisation as epithelial cell layers, we also discuss biochips for culturing endothelial cell layers. Furthermore, we review approaches to integrate tools to analyse and manipulate epithelia and endothelia in microfluidic biochips; including methods to perform electrical impedance spectroscopy; methods to detect substances undergoing trans-epithelial transport via fluorescence, spectrophotometry, and mass spectrometry; techniques to mechanically stimulate cells via stretching and fluid flow-induced shear stress; and methods to carry out high-resolution imaging of vesicular trafficking using light microscopy. Taken together, this versatile microfluidic toolbox enables novel experimental approaches to characterise epithelial monolayers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.