ArticlePloS one2014
Characterization and mutational analysis of omega-class GST (GSTO1) from Apis cerana cerana, a gene involved in response to oxidative stress.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Chromosomal inversions modulate 3D chromatin interactions and regulatory architecture inResearch square · 2026Article
- Structural and Functional Insights into a Honey Bee Omega-Class Glutathione S-Transferase Mediating Chemical Sequestration and Antioxidative Stress.Journal of agricultural and food chemistry · 2026Article
- Unraveling the functional dynamics of Caenorhabditis elegans stress-responsive omega class GST-44.The FEBS journal · 2025Article
- Mechanisms of Pathogen and Pesticide Resistance in Honey Bees.Physiology (Bethesda, Md.) · 2024Review
- A dominance of Mu class glutathione transferases within the equine tapewormParasitology · 2024Article
- Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants.Experimental & applied acarology · 2022Article
- Identification of an inositol-3-phosphate synthase 1-B gene (AccIPS1-B) from Apis cerana cerana and its role in abiotic stress.Cell stress & chaperones · 2019Article
- RNA-seq reveals disruption of gene regulation when honey bees are caged and deprived of hive conditions.The Journal of experimental biology · 2019Article
- Role of AccMGST1 in oxidative stress resistance in Apis cerana cerana.Cell stress & chaperones · 2019Article
- The Roles of Four Novel P450 Genes in Pesticides Resistance inFrontiers in genetics · 2019Article
- Molecular mechanism by whichBioscience reports · 2018Article
- Identification of a melatonin receptor type 1A gene (AccMTNR1A) in Apis cerana cerana and its possible involvement in the response to low temperature stress.Die Naturwissenschaften · 2018Article
- De novo transcriptomic analysis of the alimentary tract of the tephritid gall fly, Procecidochares utilis.PloS one · 2018Article
- The Wisdom of Honeybee Defenses Against Environmental Stresses.Frontiers in microbiology · 2018Review
- Identification and Characterization of Three New Cytochrome P450 Genes and the Use of RNA Interference to Evaluate Their Roles in Antioxidant Defense inFrontiers in physiology · 2018Article
- Identification, Expression, and Regulation of an Omega Class Glutathione S-transferase inFrontiers in physiology · 2018Article
- Characterization of the CDK5 gene in Apis cerana cerana (AccCDK5) and a preliminary identification of its activator gene, AccCDK5r1.Cell stress & chaperones · 2018Article
- Differences in the sensitivity of ovarian cancer to photodynamic therapy and the mechanisms for those differences.Oncology letters · 2017Article
- A typical RNA-binding protein gene (AccRBM11) in Apis cerana cerana: characterization of AccRBM11 and its possible involvement in development and stress responses.Cell stress & chaperones · 2016Article
- A glutathione S-transferase gene associated with antioxidant properties isolated from Apis cerana cerana.Die Naturwissenschaften · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Omega-class of GSTs (GSTOs) is a class of cytosolic GSTs that have specific structural and functional characteristics that differ from those of other GST groups. In this study, we demonstrated the involvement of the GSTO1 gene from A. cerana cerana in the oxidative stress response and further investigated the effects of three cysteine residues of GSTO1 protein on this response. Real-time quantitative PCR (qPCR) showed that AccGSTO1 was highly expressed in larvae and foragers, primarily in the midgut, epidermis, and flight muscles. The AccGSTO1 mRNA was significantly induced by cold and heat at 1 h and 3 h. The TBA (2-Thiobarbituric acid) method indicated that cold or heat resulted in MDA accumulation, but silencing of AccGSTO1 by RNAi in honeybees increased the concentration of MDA. RNAi also increased the temperature sensitivity of honeybees and markedly reduced their survival. Disc diffusion assay indicated that overexpression of AccGSTO1 in E. coli caused the resistance to long-term oxidative stress. Furthermore, AccGSTO1 was active in an in vitro DNA protection assay. Mutations in Cys-28, Cys-70, and Cys-124 affected the catalytic activity and antioxidant activity of AccGSTO1. The predicted three-dimensional structure of AccGSTO1 was also influenced by the replacement of these cysteine residues. These findings suggest that AccGSTO1 plays a protective role in the response to oxidative stress.
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