ArticleScientific reports2014
Super-resolution microscopy reveals decondensed chromatin structure at transcription sites.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Active processes shape and move the genome and nucleoplasm.Current opinion in genetics & development · 2026Review
- Histopathological effects of the fruit extract of Citrullus colocynthis on the integument of the female tick Hyalomma dromedarii.Scientific reports · 2026Article
- Transcription-dependent mobility of single genes and genome-wide motions in live human cells.Nature communications · 2024Article
- KSHV Topologically Associating Domains in Latent and Reactivated Viral Chromatin.Journal of virology · 2022Article
- H1.0 C Terminal Domain Is Integral for Altering Transcription Factor Binding within Nucleosomes.Biochemistry · 2022Article
- A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM.Nature methods · 2021Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Chromatin hierarchical branching visualized at the nanoscale by electron microscopy.Nanoscale advances · 2021Article
- Article
- Sublethal concentrations of acetylcarvacrol affect reproduction and integument morphology in the brown dog tick Rhipicephalus sanguineus sensu lato (Acari: Ixodidae).Experimental & applied acarology · 2020Article
- Infrared nanospectroscopic mapping of a single metaphase chromosome.Nucleic acids research · 2019Article
- The transition structure of chromatin fibers at the nanoscale probed by cryogenic electron tomography.Nanoscale · 2019Article
- Principles of genome folding into topologically associating domains.Science advances · 2019Review
- Mechanisms for the epigenetic inheritance of stress response in single cells.Current genetics · 2018Review
- Formation of correlated chromatin domains at nanoscale dynamic resolution during transcription.Nucleic acids research · 2018Article
- Streamlined histone-based fluorescence lifetime imaging microscopy (FLIM) for studying chromatin organisation.Biology open · 2018Article
- Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.Nature cell biology · 2017Article
- Orientation and repositioning of chromosomes correlate with cell geometry-dependent gene expression.Molecular biology of the cell · 2017Article
- Superresolution imaging of nanoscale chromosome contacts.Scientific reports · 2017Article
- Matrix mechanics controls FHL2 movement to the nucleus to activate p21 expression.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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4 authors.
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Abstract
Remodeling of the local chromatin structure is essential for the regulation of gene expression. While a number of biochemical and bioimaging experiments suggest decondensed chromatin structures are associated with transcription, a direct visualization of DNA and transcriptionally active RNA polymerase II (RNA pol II) at super-resolution is still lacking. Here we investigate the structure of chromatin isolated from HeLa cells using binding activatable localization microscopy (BALM). The sample preparation method preserved the structural integrity of chromatin. Interestingly, BALM imaging of the chromatin spreads revealed the presence of decondensed chromatin as gap structures along the spreads. These gaps were enriched with phosphorylated S5 RNA pol II, and were sensitive to the cellular transcriptional state. Taken together, we could visualize the decondensed chromatin regions together with active RNA pol II for the first time using super-resolution microscopy.
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