Evidence map›Paper›PMID 24667262›Full record

ArticleZhongguo fei ai za zhi = Chinese journal of lung cancer2014

[High resolution melting curve method for detection of BIM deletion].

Jinjing Xia, Hao Bai, Liwen Xiong, Rong Li, Bo Yan, Minhua Shao, Baohui Han

Open access · greenAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Zhongguo fei ai za zhi = Chinese journal of lung cancer, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jinjing XiaDepartment of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University, 
Shanghai 200030, China.
Hao BaiDepartment of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University, 
Shanghai 200030, China.
Liwen XiongDepartment of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University, 
Shanghai 200030, China.
Rong LiDepartment of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University, 
Shanghai 200030, China.
Bo YanDepartment of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University, 
Shanghai 200030, China.
Minhua ShaoDepartment of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University, 
Shanghai 200030, China.
Baohui HanDepartment of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University, 
Shanghai 200030, China.
Shanghai Chest Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aim of this study is to establish a HRM (high resolution melting curve) method for detection of deletion in human BIM gene and to detect this site deletion with the above method in 30 lung cancer samples and 30 normal samples.

methodsThe primers for detection of BIM deletion were designed and synthesized. The HRM method for geng deletion was established. And select the part of samples to detect BIM delection by normal PCR and sequencing assay. The Tm value of wild type PCR products was higher than that of the deletion PCR products. The difference of the corresponding Tm value is 2.5 oC.

resultsBy detection with HRM methods, 1 samples were confirmed to be mutant, 7 samples were confirmed to be heterozygous and the other 22 samples were all wild type in the lung cancer samples. 2 samples were confirmed to be heterozygous and the other 28 samples were all wild type in the normal samples.

conclusionsThe HRM method for detection of BIM deletion established in this study is a sensitive, accurate, simple and high throughput method.

Indexed as

Genetic TechniquesSequence DeletionApoptosis Regulatory ProteinsBase SequenceBcl-2-Like Protein 11HumansLung NeoplasmsMembrane ProteinsMolecular Sequence DataMutationPolymerase Chain ReactionProto-Oncogene ProteinsTransition TemperatureApoptosis Regulatory ProteinsBCL2L11 protein, humanBcl-2-Like Protein 11Membrane ProteinsProto-Oncogene Proteins

Identifiers

PMID24667262
PMCPMC6019364
OpenAlexW1600321152

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.