ArticleMolecular microbiology2014
Metal specificity of cyanobacterial nickel-responsive repressor InrS: cells maintain zinc and copper below the detection threshold for InrS.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Bacterial Metallostasis: Metal Sensing, Metalloproteome Remodeling, and Metal Trafficking.Chemical reviews · 2024Review
- Article
- Nickel Metalloregulators and Chaperones.Inorganics · 2019Article
- Bacterial zinc uptake regulator proteins and their regulons.Biochemical Society transactions · 2018Review
- An XAS investigation of the nickel site structure in the transcriptional regulator InrS.Journal of inorganic biochemistry · 2017Article
- Fine control of metal concentrations is necessary for cells to discern zinc from cobalt.Nature communications · 2017Article
- Transcriptomic analysis of nickel exposure in Sphingobium sp. ba1 cells using RNA-seq.Scientific reports · 2017Article
- The Metal Drives the Chemistry: Dual Functions of Acireductone Dioxygenase.Chemical reviews · 2017Review
- Glutamate Ligation in the Ni(II)- and Co(II)-Responsive Escherichia coli Transcriptional Regulator, RcnR.Inorganic chemistry · 2017Article
- A tight tunable range for Ni(II) sensing and buffering in cells.Nature chemical biology · 2017Article
- The mechanism of a formaldehyde-sensing transcriptional regulator.Scientific reports · 2016Article
- The Effectors and Sensory Sites of Formaldehyde-responsive Regulator FrmR and Metal-sensing Variant.The Journal of biological chemistry · 2016Article
- Generating a Metal-responsive Transcriptional Regulator to Test What Confers Metal Sensing in Cells.The Journal of biological chemistry · 2015Article
- Metals in cyanobacteria: analysis of the copper, nickel, cobalt and arsenic homeostasis mechanisms.Life (Basel, Switzerland) · 2014Review
- Metal preferences and metallation.The Journal of biological chemistry · 2014Review
- Cu(I)-mediated allosteric switching in a copper-sensing operon repressor (CsoR).The Journal of biological chemistry · 2014Article
- Global transcriptional profiles of the copper responses in the cyanobacterium Synechocystis sp. PCC 6803.PloS one · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
InrS is a Ni(II)-responsive, CsoR/RcnR-like, DNA-binding transcriptional repressor of the nrsD gene, but the Ni(II) co-ordination sphere of InrS is unlike Ni(II)-RcnR. We show that copper and Zn(II) also bind tightly to InrS and in vitro these ions also impair InrS binding to the nrsD operator-promoter. InrS does not respond to Zn(II) (or copper) in vivo after 48 h, when Zn(II) sensor ZiaR responds, but InrS transiently responds (1 h) to both metals. InrS conserves only one (of two) second co-ordination shell residues of CsoR (Glu98 in InrS). The allosteric mechanism of InrS is distinct from Cu(I)-CsoR and conservation of deduced second shell residues better predicts metal specificity than do the metal ligands. The allosteric mechanism of InrS permits greater promiscuity in vitro than CsoR. The factors dictating metal-selectivity in vivo are that KNi(II) and ΔG(C)(Ni(II)-InrS·DNA) are sufficiently high, relative to other metal sensors, for InrS to detect Ni(II), while the equivalent parameters for copper may be insufficient for copper-sensing in Synechocystis (at 48 h). InrS K(Zn(II)) (5.6 × 10(-13) M) is comparable to the sensory sites of ZiaR (and Zur), but ΔG(C)(Zn(II)-InrS·DNA) is less than ΔG(C)(Zn(II)-ZiaR·DNA) implying that relative to other sensors, ΔG(C)(Zn(II)-Sensor·DNA) rather than K(Zn(II)) determines the final detection threshold for Zn(II).
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