ArticleInfection and immunity2014
Clostridium difficile extracytoplasmic function σ factor σV regulates lysozyme resistance and is necessary for pathogenesis in the hamster model of infection.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 51 citations in OpenAlex.
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- The role of site-2-proteases in bacteria: a review on physiology, virulence, and therapeutic potential.microLife · 2023Review
- The WalRK Two-Component System Is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile.Journal of bacteriology · 2022Article
- Activation of the Extracytoplasmic Function σ Factor σmSphere · 2022Article
- Activation of the extracytoplasmic function σ factor σCurrent opinion in microbiology · 2022Review
- Cationic Homopolymers Inhibit Spore and Vegetative Cell Growth ofACS infectious diseases · 2021Article
- Article
- Peptidoglycan analysis reveals that synergistic deacetylase activity in vegetativeThe Journal of biological chemistry · 2020Article
- Lysozyme Resistance in Clostridioides difficile Is Dependent on Two Peptidoglycan Deacetylases.Journal of bacteriology · 2020Article
- Effect of Lipidation on the Localization and Activity of a Lysozyme Inhibitor inJournal of bacteriology · 2020Article
- Changes in the expression of genes encoding type IV pili-associated proteins are seen when Clostridium perfringens is grown in liquid or on surfaces.BMC genomics · 2020Article
- Activation of the Extracytoplasmic Function σ Factor σmSphere · 2019Article
- The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile.Infection and immunity · 2019Article
- Activation of the extracytoplasmic function σ factor σMolecular microbiology · 2019Review
- Impact of CodY protein on metabolism, sporulation and virulence in Clostridioides difficile ribotype 027.PloS one · 2019Article
- Multiple factors contribute to bimodal toxin gene expression in Clostridioides (Clostridium) difficile.Molecular microbiology · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Clostridium difficile is a clinically important pathogen and the most common cause of hospital-acquired infectious diarrhea. Expression of the C. difficile gene csfV, which encodes σ(V), an extracytoplasmic function σ factor, is induced by lysozyme, which damages the peptidoglycan of bacteria. Here we show that σ(V) is required for lysozyme resistance in C. difficile. Using microarray analysis, we identified the C. difficile genes whose expression is dependent upon σ(V) and is induced by lysozyme. Although the peptidoglycan of wild-type C. difficile is intrinsically highly deacetylated, we have found that exposure to lysozyme leads to additional peptidoglycan deacetylation. This lysozyme-induced deacetylation is dependent upon σ(V). Expression of pdaV, which encodes a putative peptidoglycan deacetylase, was able to increase lysozyme resistance of a csfV mutant. The csfV mutant strain is severely attenuated compared to wild-type C. difficile in a hamster model of C. difficile-associated disease. We conclude that the σ(V) signal transduction system, which senses the host innate immune defense enzyme lysozyme, is required for lysozyme resistance and is necessary during C. difficile infection.
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