ArticlePloS one2014
Internal transcribed spacer 1 secondary structure analysis reveals a common core throughout the anaerobic fungi (Neocallimastigomycota).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Advances in Lignocellulose-Degrading Enzyme Discovery from Anaerobic Rumen Fungi.Microorganisms · 2025Review
- Fecal microbiota colonization dynamics in dairy heifers associated with early-life rumen microbiota modulation and gut health.Frontiers in microbiology · 2024Article
- Influence of scat ageing on the gut microbiome: how old is too old?BMC genomics · 2023Article
- Methods used for the study of endophytic fungi: a review on methodologies and challenges, and associated tips.Archives of microbiology · 2022Review
- The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem.Animals : an open access journal from MDPI · 2022Article
- Long-term effects of early-life rumen microbiota modulation on dairy cow production performance and methane emissions.Frontiers in microbiology · 2022Article
- Effects of long-acting, broad spectra anthelmintic treatments on the rumen microbial community compositions of grazing sheep.Scientific reports · 2021Article
- The Effect of a High-Grain Diet on the Rumen Microbiome of Goats with a Special Focus on Anaerobic Fungi.Microorganisms · 2021Article
- Fresh Rumen Liquid Inoculant Enhances the Rumen Microbial Community Establishment in Pre-weaned Dairy Calves.Frontiers in microbiology · 2021Article
- Early-diverging fungal phyla: taxonomy, species concept, ecology, distribution, anthropogenic impact, and novel phylogenetic proposals.Fungal diversity · 2021Review
- Article
- Seasonal and Nutrient Supplement Responses in Rumen Microbiota Structure and Metabolites of Tropical Rangeland Cattle.Microorganisms · 2020Article
- A potential alternative to traditional antibiotics in aquaculture: Yeast glycoprotein exhibits antimicrobial effect in vivo and in vitro on Aeromonas caviae isolated from Carassius auratus gibelio.Veterinary medicine and science · 2020Article
- Comparison of Rumen Microbiota and Serum Biochemical Indices in White Cashmere Goats Fed Ensiled or Sun-Dried Mulberry Leaves.Microorganisms · 2020Article
- Domesticated equine species and their derived hybrids differ in their fecal microbiota.Animal microbiome · 2020Article
- Nucleotide and protein researches on anaerobic fungi during four decades.Journal of animal science and technology · 2020Review
- Multi-kingdom characterization of the core equine fecal microbiota based on multiple equine (sub)species.Animal microbiome · 2020Article
- Anaerobic Fungi: Past, Present, and Future.Frontiers in microbiology · 2020Review
- A heritable subset of the core rumen microbiome dictates dairy cow productivity and emissions.Science advances · 2019Article
- A highly efficient electrophoretic method for discrimination between two Neoscytalidium species using a specific fungal internal transcribed spacer (ITS) fragment.Folia microbiologica · 2019Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The internal transcribed spacer (ITS) is a popular barcode marker for fungi and in particular the ITS1 has been widely used for the anaerobic fungi (phylum Neocallimastigomycota). A good number of validated reference sequences of isolates as well as a large number of environmental sequences are available in public databases. Its highly variable nature predisposes the ITS1 for low level phylogenetics; however, it complicates the establishment of reproducible alignments and the reconstruction of stable phylogenetic trees at higher taxonomic levels (genus and above). Here, we overcame these problems by proposing a common core secondary structure of the ITS1 of the anaerobic fungi employing a Hidden Markov Model-based ITS1 sequence annotation and a helix-wise folding approach. We integrated the additional structural information into phylogenetic analyses and present for the first time an automated sequence-structure-based taxonomy of the ITS1 of the anaerobic fungi. The methodology developed is transferable to the ITS1 of other fungal groups, and the robust taxonomy will facilitate and improve high-throughput anaerobic fungal community structure analysis of samples from various environments.
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