ArticleGenome research2014
Identifying mRNA sequence elements for target recognition by human Argonaute proteins.
Article in Genome research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Centrosome‑, mitotic spindle‑ and cytokinetic bridge‑specific compartmentalization of AGO2 protein in human liver cells undergoing mitosis: Non‑canonical, RNAi‑dependent, control of local homeostasis.Molecular medicine reports · 2025Article
- Argonaute 2 Restores Erectile Function by Enhancing Angiogenesis and Reducing Reactive Oxygen Species Production in Streptozotocin (STZ)-Induced Type-1 Diabetic Mice.International journal of molecular sciences · 2023Article
- A deep learning method for miRNA/isomiR target detection.Scientific reports · 2022Article
- tRForest: a novel random forest-based algorithm for tRNA-derived fragment target prediction.NAR genomics and bioinformatics · 2022Article
- Needles in Haystacks: Understanding the Success of Selective Pairing of Nucleic Acids.International journal of molecular sciences · 2022Article
- The Importance of AGO 1 and 4 in Post-Transcriptional Gene Regulatory Function of tRF5-GluCTC, an Respiratory Syncytial Virus-Induced tRNA-Derived RNA Fragment.International journal of molecular sciences · 2020Article
- AGO2 MediatesMolecular cancer research : MCR · 2020Article
- AGO unchained: Canonical and non-canonical roles of Argonaute proteins in mammals.Frontiers in bioscience (Landmark edition) · 2020Review
- Transcription Factors Targeted by miRNAs Regulating Smooth Muscle Cell Growth and Intimal Thickening after Vascular Injury.International journal of molecular sciences · 2019Review
- Argonaute2 attenuates active transcription by limiting RNA Polymerase II elongation in Drosophila melanogaster.Scientific reports · 2018Article
- Prediction of RNA-protein sequence and structure binding preferences using deep convolutional and recurrent neural networks.BMC genomics · 2018Article
- A deep boosting based approach for capturing the sequence binding preferences of RNA-binding proteins from high-throughput CLIP-seq data.Nucleic acids research · 2017Article
- WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms.Nucleic acids research · 2017Article
- Expression Status of PIWIL1 as a Prognostic Marker of Colorectal Cancer.Disease markers · 2017Article
- TarPmiR: a new approach for microRNA target site prediction.Bioinformatics (Oxford, England) · 2016Article
- Learning to Predict miRNA-mRNA Interactions from AGO CLIP Sequencing and CLASH Data.PLoS computational biology · 2016Article
- A deep learning framework for modeling structural features of RNA-binding protein targets.Nucleic acids research · 2016Article
- The primate-specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming.Nature genetics · 2016Article
- Specificity and nonspecificity in RNA-protein interactions.Nature reviews. Molecular cell biology · 2015Review
- ComiRNet: a web-based system for the analysis of miRNA-gene regulatory networks.BMC bioinformatics · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is commonly known that mammalian microRNAs (miRNAs) guide the RNA-induced silencing complex (RISC) to target mRNAs through the seed-pairing rule. However, recent experiments that coimmunoprecipitate the Argonaute proteins (AGOs), the central catalytic component of RISC, have consistently revealed extensive AGO-associated mRNAs that lack seed complementarity with miRNAs. We herein test the hypothesis that AGO has its own binding preference within target mRNAs, independent of guide miRNAs. By systematically analyzing the data from in vivo cross-linking experiments with human AGOs, we have identified a structurally accessible and evolutionarily conserved region (∼10 nucleotides in length) that alone can accurately predict AGO-mRNA associations, independent of the presence of miRNA binding sites. Within this region, we further identified an enriched motif that was replicable on independent AGO-immunoprecipitation data sets. We used RNAcompete to enumerate the RNA-binding preference of human AGO2 to all possible 7-mer RNA sequences and validated the AGO motif in vitro. These findings reveal a novel function of AGOs as sequence-specific RNA-binding proteins, which may aid miRNAs in recognizing their targets with high specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.