Evidence map›Paper›PMID 24663124›Full record

ArticlePloS one2014

Prostaglandin (PG) F2 alpha synthesis in human subcutaneous and omental adipose tissue: modulation by inflammatory cytokines and role of the human aldose reductase AKR1B1.

Andréanne Michaud, Nicolas Lacroix-Pépin, Mélissa Pelletier, Alain Veilleux, Suzanne Noël, Céline Bouchard, Picard Marceau, Michel A Fortier, André Tchernof

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 29 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Andréanne MichaudEndocrinology and Nephrology, Laval University Medical Center, Quebec City, Canada; Department of Nutrition, Laval University, Quebec City, Canada.
Nicolas Lacroix-PépinReproduction and Biology, Laval University Medical Center, Quebec City, Canada.
Mélissa PelletierEndocrinology and Nephrology, Laval University Medical Center, Quebec City, Canada.
Alain VeilleuxDepartment of Nutrition, Laval University, Quebec City, Canada.
Suzanne NoëlGynecology Unit, Laval University Medical Center, Quebec City, Canada.
Céline BouchardGynecology Unit, Laval University Medical Center, Quebec City, Canada.
Picard MarceauDepartment of Surgery, Quebec Cardiology and Pulmonology Institute, Quebec City, Canada.
Michel A FortierReproduction and Biology, Laval University Medical Center, Quebec City, Canada.
André TchernofEndocrinology and Nephrology, Laval University Medical Center, Quebec City, Canada; Department of Nutrition, Laval University, Quebec City, Canada.
Université Laval · CAInstitut Universitaire de Cardiologie et de Pneumologie de Québec · CA

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

introductionPGF2α may be involved in the regulation of adipose tissue function.

objectives1) To examine PGF2α release by primary preadipocytes, mature adipocytes and whole tissue explants from the subcutaneous and omental fat compartments; 2) To assess which PGF synthase is the most relevant in human adipose tissue.

methodsFat samples were obtained by surgery in women. PGF2α release by preadipocytes, adipocytes and explants under stimulation by TNF-α, IL-1β or both was measured. Messenger RNA expression levels of AKR1B1 and AKR1C3 were measured by RT-PCR in whole adipose tissue and cytokine-treated preadipocytes. The effect of AKR1B1 inhibitor ponalrestat on PGF2α synthesis was investigated.

resultsPGF2α release was significantly induced in response to cytokines compared to control in omental (p = 0.01) and to a lesser extent in subcutaneous preadipocytes (p = 0.02). Messenger RNA of COX-2 was significantly higher in omental compared to subcutaneous preadipocytes in response to combined TNF-α and IL-1β (p = 0.01). Inflammatory cytokines increased AKR1B1 mRNA expression and protein levels (p≤0.05), but failed to increase expression levels of AKR1C3 in cultured preadipocytes. Accordingly, ponalrestat blunted PGF2α synthesis by preadipocytes in basal and stimulated conditions (p≤0.05). Women with the highest PGF2α release by omental adipocytes had a higher BMI (p = 0.05), waist circumference (p≤0.05) and HOMAir index (p≤0.005) as well as higher mRNA expression of AKR1B1 in omental (p<0.10) and subcutaneous (p≤0.05) adipose tissue compared to women with low omental adipocytes PGF2α release. Positive correlations were observed between mRNA expression of AKR1B1 in both compartments and BMI, waist circumference as well as HOMAir index (p≤0.05 for all).

conclusionPGF2α release by omental mature adipocytes is increased in abdominally obese women. Moreover, COX-2 expression and PGF2α release is particularly responsive to inflammatory stimulation in omental preadipocytes. Yet, blockade of PGF synthase AKR1B1 inhibits most of the PGF2α release.

Indexed as

AdipocytesAdultAldehyde ReductaseCyclooxygenase 2CytokinesDinoprostEnzyme InhibitorsFemaleGene Expression Regulation, EnzymologicGlucoseHomeostasisHumansInflammationMacrophagesMiddle AgedObesityAKR1B1 protein, humanAldehyde ReductaseCyclooxygenase 2CytokinesDinoprostEnzyme InhibitorsGlucose

Identifiers

PMID24663124
PMCPMC3963845
OpenAlexW2061687045

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.