ArticleScientific reports2014
Hydrogel microfluidics for the patterning of pluripotent stem cells.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Investigating the coupled effects of stiffness and stretch on the trabecular meshwork cells using a hydrogel-integrated microfluidic system.Biomaterials science · 2026Article
- Artificial capillaries-on-a-chip with modular control over lumen size, architecture, and co-culture conditions.Biofabrication · 2026Article
- Transforming Fragile Hydrogel Chips Into Standardized Cartridges via Contact Line Pinning for Robust Microfluidics.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Characterizing the Impact of Fabrication Methods on Mechanically Tunable Gelatin Hydrogels for Cardiac Fibrosis Studies.Bioengineering (Basel, Switzerland) · 2025Article
- Bioengineering innovations for neural organoids with enhanced fidelity and function.Cell stem cell · 2025Review
- Preparation and Mechano-Functional Characterization of PEGylated Fibrin Hydrogels: Impact of Thrombin Concentration.Gels (Basel, Switzerland) · 2024Article
- Patterning ganglionic eminences in developing human brain organoids using a morphogen-gradient-inducing device.Cell reports methods · 2024Article
- Cell type specification and diversity in subpallial organoids.Frontiers in genetics · 2024Review
- A Silicon-Based PDMS-PEG Copolymer Microfluidic Chip for Real-Time Polymerase Chain Reaction Diagnosis.Journal of functional biomaterials · 2023Article
- Review
- Differential Single Cell Responses of Embryonic Stem Cells Versus Embryoid Bodies to Gravity Mechanostimulation.Stem cells and development · 2022Article
- Application of microfluidic chips in anticancer drug screening.Bosnian journal of basic medical sciences · 2022Review
- Bioengineering the human spinal cord.Frontiers in cell and developmental biology · 2022Review
- Cancer Stem Cells in Tumor Modeling: Challenges and Future Directions.Advanced nanobiomed research · 2021Article
- Microfluidics as efficient technology for the isolation and characterization of stem cells.EXCLI journal · 2021Review
- Recent Advancements in Engineering Strategies for Manipulating Neural Stem Cell Behavior.Current tissue microenvironment reports · 2020Article
- Layer-by-layer fabrication of 3D hydrogel structures using open microfluidics.Lab on a chip · 2020Article
- Injection Molded Microfluidics for Establishing High-Density Single Cell Arrays in an Open Hydrogel Format.Analytical chemistry · 2020Article
- A Fiber Alginate Co-culture Platform for the Differentiation of mESC and Modeling of the Neural Tube.Frontiers in neuroscience · 2020Article
- Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three-Dimensional Hydrogels.Current protocols in stem cell biology · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biomolecular signaling is of utmost importance in governing many biological processes such as the patterning of the developing embryo where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly controlled fashion. Although state-of-the-art microfluidic technologies allow precise biomolecule delivery in time and space, long-term (stem) cell culture at the micro-scale is often far from ideal due to medium evaporation, limited space for cell growth or shear stress. To overcome these challenges, we here introduce a concept based on hydrogel microfluidics for decoupling conventional, macro-scale cell culture from precise biomolecule delivery through a gel layer. We demonstrate the spatiotemporally controlled neuronal commitment of mouse embryonic stem cells via delivery of retinoic acid gradients. This technique should be useful for testing the effect of dose and timing of biomolecules, singly or in combination, on stem cell fate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.