ArticleThe Journal of biological chemistry2014
Distinct modes of ubiquitination of peroxisome-targeting signal type 1 (PTS1) receptor Pex5p regulate PTS1 protein import.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Polyunsaturated fatty acid metabolism in the retinal pigment epithelium and its association with outer retinal disease.Mammalian genome : official journal of the International Mammalian Genome Society · 2026Review
- Structural basis of receptor retro-translocation in peroxisomal protein import.bioRxiv : the preprint server for biology · 2026Article
- Pex6 and ubiquitination regulate topological remodeling of the peroxisomal membrane protein Pex14.The Journal of biological chemistry · 2026Article
- Identifying and characterizing a missing peroxin-PEX8-in Arabidopsis thaliana.The Plant cell · 2025Article
- Peroxisomal homeostasis in metabolic diseases and its implication in ferroptosis.Cell communication and signaling : CCS · 2024Review
- The peroxisome: an update on mysteries 3.0.Histochemistry and cell biology · 2024Review
- The Cys-N-degron pathway modulates pexophagy through the N-terminal oxidation and arginylation of ACAD10.Autophagy · 2023Article
- Article
- Organelle Engineering in Yeast: Enhanced Production of Protopanaxadiol through Manipulation of Peroxisome Proliferation inMicroorganisms · 2022Article
- Non-lysine ubiquitylation: Doing things differently.Frontiers in molecular biosciences · 2022Review
- Peroxisome Deficiency Impairs BDNF Signaling and Memory.Frontiers in cell and developmental biology · 2020Article
- Peroxisome: Metabolic Functions and Biogenesis.Advances in experimental medicine and biology · 2020Review
- Dual role of USP30 in controlling basal pexophagy and mitophagy.EMBO reports · 2018Article
- New splicing variants of mitochondrial Rho GTPase-1 (Miro1) transport peroxisomes.The Journal of cell biology · 2018Article
- Redox Regulation of Homeostasis and Proteostasis in Peroxisomes.Physiological reviews · 2018Review
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
- TRIM37, a novel E3 ligase for PEX5-mediated peroxisomal matrix protein import.The Journal of cell biology · 2017Article
- Role of PEX5 ubiquitination in maintaining peroxisome dynamics and homeostasis.Cell cycle (Georgetown, Tex.) · 2017Review
- Genetic Interactions between PEROXIN12 and Other Peroxisome-Associated Ubiquitination Components.Plant physiology · 2016Article
- Autophagic degradation of peroxisomes in mammals.Biochemical Society transactions · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peroxisome targeting signal type-1 (PTS1) receptor, Pex5p, is a key player in peroxisomal matrix protein import. Pex5p recognizes PTS1 cargoes in the cytosol, targets peroxisomes, translocates across the membrane, unloads the cargoes, and shuttles back to the cytosol. Ubiquitination of Pex5p at a conserved cysteine is required for the exit from peroxisomes. However, any potential ubiquitin ligase (E3) remains unidentified in mammals. Here, we establish an in vitro ubiquitination assay system and demonstrate that RING finger Pex10p functions as an E3 with an E2, UbcH5C. The E3 activity of Pex10p is essential for its peroxisome-restoring activity, being enhanced by another RING peroxin, Pex12p. The Pex10p·Pex12p complex catalyzes monoubiquitination of Pex5p at one of multiple lysine residues in vitro, following the dissociation of Pex5p from Pex14p and the PTS1 cargo. Several lines of evidence with lysine-to-arginine mutants of Pex5p demonstrate that Pex10p RING E3-mediated ubiquitination of Pex5p is required for its efficient export from peroxisomes to the cytosol and peroxisomal matrix protein import. RING peroxins are required for both modes of Pex5p ubiquitination, thus playing a pivotal role in Pex5p shuttling.
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Registered trials
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