ArticleInternational journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases2014
Mycoplasma genitalium promotes epithelial crossing and peripheral blood mononuclear cell infection by HIV-1.
Article in International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Efficacy of Doxycycline-Sitafloxacin Sequential Therapy for Urogenital Mycoplasma genitalium Infection in Nanjing, China.Sexually transmitted diseases · 2025Article
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- Sexually Transmitted Infections in People with Human Immunodeficiency Virus.Infectious disease clinics of North America · 2024Review
- Prevalence ofOpen forum infectious diseases · 2024Article
- Article
- Prevalence and incidence ofSexually transmitted infections · 2020Article
- Experimental Infection of Pig-Tailed Macaques (Macaca nemestrina) with Mycoplasma genitalium.Infection and immunity · 2017Article
- Clinical Laboratory Assessment of Mycoplasma genitalium Transcription-Mediated Amplification Using Primary Female Urogenital Specimens.Journal of clinical microbiology · 2016Article
- Mycoplasma genitalium: An Overlooked Sexually Transmitted Pathogen in Women?Infectious diseases in obstetrics and gynecology · 2016Review
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundMycoplasma genitalium co-infection in HIV-infected individuals has been reported to increase the shedding of HIV in the urogenital region of females. To better understand this relationship, we investigated the influence of M. genitalium on the transmission and replication of HIV using an in vitro model.
methodsThe Transwell co-culture system was employed to assess the crossing of an endocervical cell barrier by HIV-1. Immunocytochemistry and confocal microscopy were used to assess the distribution of the nectin-1 molecule on M. genitalium-infected epithelial cells of the End1/E6E7 endocervical cell line, grown as monolayers in the insert wells. Peripheral blood mononuclear cells (PBMC) were cultured in the bottom wells to assess the effects of M. genitalium, passing through the semipermeable culturing membrane, on subsequent HIV infection of susceptible target cells.
resultsInfection of the endocervical cells with the adhesion-positive M. genitalium G37 strain (wild-type) significantly elevated the passage of HIV across the epithelial cell barrier relative to HIV transfer across endocervical cells infected with the adhesion-negative M. genitalium JB1 strain. Immunostaining of the M. genitalium-G37-infected epithelial cells disclosed capping and internalization of the junctional regulatory protein nectin-1, in association with reduced transepithelial resistance (TER) in the cell monolayer. When PBMC were cultured beneath insert wells containing M. genitalium-G37-infected epithelial cell monolayers, we observed significantly enhanced infectivity and replication of HIV added afterward to the cultures.
conclusionsM. genitalium influences events on both sides of a cultured mucosal epithelial monolayer: (1) by infecting the epithelial cells and reducing the integrity of the barrier itself, and (2) by activating HIV target cells below it, thereby promoting HIV infection and progeny virus production.
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