Evidence map›Paper›PMID 24661552›Full record

ArticleBiomaterials2014

Uptake and transfection efficiency of PEGylated cationic liposome-DNA complexes with and without RGD-tagging.

Ramsey N Majzoub, Chia-Ling Chan, Kai K Ewert, Bruno F B Silva, Keng S Liang, Erica L Jacovetty, Bridget Carragher, Clinton S Potter, Cyrus R Safinya

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 85 citations in OpenAlex.

  1. Liposomes as delivery vectors for nucleic acid mimics intoFrontiers in bioengineering and biotechnology · 2026
    Article
  2. Efficiency and safety of five different agents forFrontiers in molecular biosciences · 2026
    Article
  3. Review
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  11. Methods for Intracellular Delivery of Quantum Dots.Topics in current chemistry (Cham) · 2021
    Review
  12. Article
  13. Article
  14. Review
  15. Article
  16. Barriers and Strategies of Cationic Liposomes for Cancer Gene Therapy.Molecular therapy. Methods & clinical development · 2020
    Review
  17. Article
  18. Lipidoid-siRNA Nanoparticle-Mediated IL-1β Gene Silencing for Systemic Arthritis Therapy in a Mouse Model.Molecular therapy : the journal of the American Society of Gene Therapy · 2019
    Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Ramsey N MajzoubDepartment of Physics, University of California, Santa Barbara, CA 93106, USA; Department of Materials, University of California, Santa Barbara, CA 93106, USA; Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA.
Chia-Ling ChanDepartment of Physics, University of California, Santa Barbara, CA 93106, USA; Department of Materials, University of California, Santa Barbara, CA 93106, USA; Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA; Institute of Physics, Academica Sinica, Taipei 11529, Taiwan; National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
Kai K EwertDepartment of Physics, University of California, Santa Barbara, CA 93106, USA; Department of Materials, University of California, Santa Barbara, CA 93106, USA; Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA.
Bruno F B SilvaDepartment of Physics, University of California, Santa Barbara, CA 93106, USA; Department of Materials, University of California, Santa Barbara, CA 93106, USA; Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA; Division of Physical Chemistry, Centre for Chemistry and Chemical Engineering, Lund University, SE-221 00 Lund, Sweden.
Keng S LiangNational Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan; Department of Electrophysics, National Chiao-Tung University, Hsinchu 30010, Taiwan.
Erica L JacovettyNational Resource for Automated Molecular Microscopy, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Bridget CarragherNational Resource for Automated Molecular Microscopy, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Clinton S PotterNational Resource for Automated Molecular Microscopy, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Cyrus R SafinyaDepartment of Physics, University of California, Santa Barbara, CA 93106, USA; Department of Materials, University of California, Santa Barbara, CA 93106, USA; Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA. Electronic address: safinya@mrl.ucsb.edu.
University of California, Santa Barbara · USScripps Research Institute · USNational Synchrotron Radiation Research Center · TW

Funding

TRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2012 to 2021
$14.7M
Structures of Lipid-DNA Nonviral Gene Delivery Systems 4R01GM059288 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI SAFINYA, CYRUS R · 1999 to 2016
$4.2M
NIGMS NIH HHS GM-59288NIGMS NIH HHS P41 GM103310NIGMS NIH HHS R01 GM059288
6 · The paper itself

Abstract

Steric stabilization of cationic liposome-DNA (CL-DNA) complexes is required for in vivo applications such as gene therapy. PEGylation (PEG: poly(ethylene glycol)) of CL-DNA complexes by addition of PEG2000-lipids yields sterically stabilized nanoparticles but strongly reduces their gene delivery efficacy. PEGylation-induced weakening of the electrostatic binding of CL-DNA nanoparticles to cells (leading to reduced uptake) has been considered as a possible cause, but experimental results have been ambiguous. Using quantitative live-cell imaging in vitro, we have investigated cell attachment and uptake of PEGylated CL-DNA nanoparticles with and without a custom synthesized RGD-peptide grafted to the distal ends of PEG2000-lipids. The RGD-tagged nanoparticles exhibit strongly increased cellular attachment as well as uptake compared to nanoparticles without grafted peptide. Transfection efficiency of RGD-tagged PEGylated CL-DNA NPs increases by about an order of magnitude between NPs with low and high membrane charge density (σM; the average charge per unit area of the membrane; controlled by the molar ratio of cationic to neutral lipid), even though imaging data show that uptake of RGD-tagged particles is only slightly enhanced by high σM. This suggests that endosomal escape and, as a result, transfection efficiency of RGD-tagged NPs is facilitated by high σM. We present a model describing the interactions between PEGylated CL-DNA nanoparticles and the anionic cell membrane which shows how the PEG grafting density and membrane charge density affect adhesion of nanoparticles to the cell surface.

Indexed as

TransfectionAnimalsCationsCell AdhesionCell LineDNAGenetic TherapyLipidsLiposomesMiceMicroscopy, ElectronNanoparticlesOligopeptidesPolyethylene GlycolsStatic Electricityarginyl-glycyl-aspartic acidCationsDNALipidsLiposomesOligopeptidesPolyethylene GlycolsGene therapyLiposomeLive cell imagingNanoparticlePolyethylene glycolRGD peptide

Identifiers

PMID24661552
PMCPMC4032065
OpenAlexW2025320888

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.