ArticleFEMS microbiology letters2014
Antibacterial enzymes from the functional screening of metagenomic libraries hosted in Ralstonia metallidurans.
Article in FEMS microbiology letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- MiGPC: a comprehensive catalog of enzybiotics from environmental metagenomes.Scientific reports · 2026Article
- Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity.Microbial cell factories · 2022Article
- Two-step functional screen on multiple proteinaceous substrates reveals temperature-robust proteases with a broad-substrate range.Applied microbiology and biotechnology · 2021Article
- Mining of two novel aldehyde dehydrogenases (DHY-SC-VUT5 and DHY-G-VUT7) from metagenome of hydrocarbon contaminated soils.BMC biotechnology · 2021Article
- Functional Metagenomic Screening for Antimicrobial Resistance in the Oral Microbiome.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Metagenomics Approaches in Discovery and Development of New Bioactive Compounds from Marine Actinomycetes.Current microbiology · 2020Review
- Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome.eLife · 2019Article
- Antibacterial and anticancer activities of orphan biosynthetic gene clusters from Atlantis II Red Sea brine pool.Microbial cell factories · 2019Article
- Article
- Boosting Secondary Metabolite Production and Discovery through the Engineering of Novel Microbial Biosensors.BioMed research international · 2018Review
- Rapid resistome mapping using nanopore sequencing.Nucleic acids research · 2017Article
- Review
- Metagenomic sequence of saline desert microbiota from wild ass sanctuary, Little Rann of Kutch, Gujarat, India.Genomics data · 2015Article
- Inhibition of the growth of Bacillus subtilis DSM10 by a newly discovered antibacterial protein from the soil metagenome.Bioengineered · 2015Article
- Biotechnological applications of functional metagenomics in the food and pharmaceutical industries.Frontiers in microbiology · 2015Review
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3 authors at 1 institution in 1 country.
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Abstract
Phenotype-based screening of bacterial metagenomic libraries provides an avenue for the discovery of novel genes, enzymes, and metabolites that have a variety of potential clinical and industrial uses. Here, we report the identification of a functionally diverse collection of antibacterially active enzymes from the phenotypic screening of 700 000 cosmid clones prepared from Arizona soil DNA and hosted in Ralstonia metallidurans. Environmental DNA clones surrounded by zones of growth inhibition in a bacterial overlay assay were found, through bioinformatics and functional analyses, to encode enzymes with predicted peptidase, lipase, and glycolytic activities conferring antibiosis. The antibacterial activities observed in our R. metallidurans-based assay could not be replicated with the same clones in screens using Escherichia coli as a heterologous host, suggesting that the large-scale screening of metagenomic libraries for antibiosis using phylogenetically diverse hosts should be a productive strategy for identifying enzymes with functionally diverse antibacterial activities.
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