ArticleACS chemical neuroscience2014
Label-free dopamine imaging in live rat brain slices.
Article in ACS chemical neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Hormonal contraceptives and the brain: A systematic review on 60 years of neuroimaging, EEG, and biochemical studies in humans and animals.Frontiers in neuroendocrinology · 2023Pooled it
- Detecting and differentiating neurotransmitters using ultraviolet plasmonic engineered native fluorescence.RSC advances · 2023Article
- Evaluation of compact pulsed lasers for two-photon microscopy using a simple method for measuring two-photon excitation efficiency.Neurophotonics · 2023Article
- Framing of Poly(arylene-ethynylene) around Carbon Nanotubes and Iodine Doping for the Electrochemical Detection of Dopamine.Biosensors · 2023Article
- The chemical tools for imaging dopamine release.Cell chemical biology · 2021Review
- Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots.Analytica chimica acta · 2021Article
- Label-free imaging of neurotransmitters in live brain tissue by multi-photon ultraviolet microscopy.Neuronal signaling · 2018Review
- Synaptic Effects of Dopamine Breakdown and Their Relation to Schizophrenia-Linked Working Memory Deficits.Frontiers in synaptic neuroscience · 2018Article
- The Fluorescence Methods to Study Neurotransmitters (Biomediators) in Plant Cells.Journal of fluorescence · 2016Review
- Effect of amyloids on the vesicular machinery: implications for somatic neurotransmission.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Exocytosis of serotonin from the neuronal soma is sustained by a serotonin and calcium-dependent feedback loop.Frontiers in cellular neuroscience · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dopaminergic neurotransmission has been investigated extensively, yet direct optical probing of dopamine has not been possible in live cells. Here we image intracellular dopamine with sub-micrometer three-dimensional resolution by harnessing its intrinsic mid-ultraviolet (UV) autofluorescence. Two-photon excitation with visible light (540 nm) in conjunction with a non-epifluorescent detection scheme is used to circumvent the UV toxicity and the UV transmission problems. The method is established by imaging dopamine in a dopaminergic cell line and in control cells (glia), and is validated by mass spectrometry. We further show that individual dopamine vesicles/vesicular clusters can be imaged in cultured rat brain slices, thereby providing a direct visualization of the intracellular events preceding dopamine release induced by depolarization or amphetamine exposure. Our technique opens up a previously inaccessible mid-ultraviolet spectral regime (excitation ~270 nm, emission < 320 nm) for label-free imaging of native molecules in live tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.