ArticleThe Journal of biological chemistry2014
Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- The role of claudins in homeostasis.Nature reviews. Nephrology · 2023Review
- Human microsporidian pathogenJournal of cell science · 2021Article
- Claudin-19 mediates the effects of NO on the paracellular pathway in thick ascending limbs.American journal of physiology. Renal physiology · 2019Article
- In-Depth Bioinformatic Study of the CLDN16 Gene and Protein: Prediction of Subcellular Localization to Mitochondria.Medicina (Kaunas, Lithuania) · 2019Article
- Rescue of tight junctional localization of a claudin-16 mutant D97S by antimalarial medicine primaquine in Madin-Darby canine kidney cells.Scientific reports · 2019Article
- Newly synthesized claudins but not occludin are added to the basal side of the tight junction.Molecular biology of the cell · 2019Article
- Phosphorylation of tight junction transmembrane proteins: Many sites, much to do.Tissue barriers · 2018Review
- A membrane fusion protein, Ykt6, regulates epithelial cell migration via microRNA-mediated suppression of Junctional Adhesion Molecule A.Cell cycle (Georgetown, Tex.) · 2018Article
- The RING finger- and PDZ domain-containing protein PDZRN3 controls localization of the MgThe Journal of biological chemistry · 2017Article
- Developmental Expression of Claudins in the Mammary Gland.Journal of mammary gland biology and neoplasia · 2017Article
- Endocytosis of tight junction proteins and the regulation of degradation and recycling.Annals of the New York Academy of Sciences · 2017Review
- Claudins and mineral metabolism.Current opinion in nephrology and hypertension · 2016Review
- Systems Proteomics View of the Endogenous Human Claudin Protein Family.Journal of proteome research · 2016Review
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Claudin-16 (CLDN16) regulates the paracellular reabsorption of Mg(2+) in the thick ascending limb of Henle's loop. However, the mechanism regulating the tight junctional localization of CLDN16 remains unknown. In yeast two-hybrid systems, we found that CLDN16 bound to syntaxin 8 (STX8), a target soluble N-ethylmaleimide-sensitive factor attachment protein receptor. We have examined the effect of STX8 on the localization and function of CLDN16 using Madin-Darby canine kidney cells expressing FLAG-tagged CLDN16. A pulldown assay showed that the carboxyl cytoplasmic region of human CLDN16 bound to STX8. CLDN16 was localized in the thick ascending limb, whereas STX8 was widely distributed throughout the rat kidney. An association between CLDN16 and STX8 was observed in rat renal homogenates and Madin-Darby canine kidney cells. STX8 siRNA decreased the cell surface localization of CLDN16 and transepithelial electrical resistance and permeability to Mg(2+) but increased the co-localization of CLDN16 with early endosome and lysosome markers. Dephosphorylation of CLDN16 by protein kinase A inhibitors and S217A mutant, a dephosphorylated form, decreased the association with STX8 and the cell surface localization of CLDN16. Recycling assays indicated that STX8 siRNA decreased the trafficking of CLDN16 to the plasma membrane without affecting endocytosis. Dominant negative Rab11 and recycling inhibitor primaquine decreased the cell surface localization of CLDN16, which was similar to that in STX8 siRNA-transfected cells. These results suggest that STX8 mediates the recycling of CLDN16 and constitutes an important component of the CLDN16 trafficking machinery in the kidney.
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