ArticleJournal of bacteriology2014
The Salmonella enterica serovar Typhi LeuO global regulator forms tetramers: residues involved in oligomerization, DNA binding, and transcriptional regulation.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- The LysR-type transcriptional regulator STY2660 is involved in outer membrane protein synthesis, bile resistance and motility inFrontiers in microbiology · 2025Article
- Functional role of 18 LysR-Type transcriptional regulators of Salmonella enterica serovar Typhi.PloS one · 2025Article
- Mechanisms regulating the CRISPR-Cas systems.Frontiers in microbiology · 2023Review
- RdsA Is a Global Regulator That Controls Cell Shape and Division inFrontiers in microbiology · 2022Article
- The CRISPR-Cas System Is Involved in OmpR Genetic Regulation for Outer Membrane Protein Synthesis inFrontiers in microbiology · 2021Article
- When is a transcription factor a NAP?Current opinion in microbiology · 2020Review
- YaeB, Expressed in Response to the Acidic pH in Macrophages, Promotes Intracellular Replication and Virulence ofInternational journal of molecular sciences · 2019Article
- Characterization of the pleiotropic LysR-type transcription regulator LeuO of Escherichia coli.Nucleic acids research · 2019Article
- Molecular basis for the differential expression of the global regulator VieA in Vibrio cholerae biotypes directed by H-NS, LeuO and quorum sensing.Molecular microbiology · 2018Article
- Regulatory Hierarchies Controlling Virulence Gene Expression inFrontiers in microbiology · 2018Review
- Vibrio cholerae LeuO Links the ToxR Regulon to Expression of Lipid A Remodeling Genes.Infection and immunity · 2016Article
- DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression.Biophysical reviews · 2016Review
- DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression.Biophysical reviews · 2016Review
- Correlation of Antagonistic Regulation ofFrontiers in cellular and infection microbiology · 2016Article
- Transcriptional analysis of the MrpJ network: modulation of diverse virulence-associated genes and direct regulation of mrp fimbrial and flhDC flagellar operons in Proteus mirabilis.Infection and immunity · 2015Article
- Integrated circuits: how transcriptional silencing and counter-silencing facilitate bacterial evolution.Current opinion in microbiology · 2015Review
- The Subtleties and Contrasts of the LeuO Regulator in Salmonella Typhi: Implications in the Immune Response.Frontiers in immunology · 2014Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
LeuO is a LysR-type transcriptional regulator (LTTR) that has been described to be a global regulator in Escherichia coli and Salmonella enterica, since it positively and negatively regulates the expression of genes involved in multiple biological processes. LeuO is comprised of an N-terminal DNA-binding domain (DBD) with a winged helix-turn-helix (wHTH) motif and of a long linker helix (LH) involved in dimerization that connects the DBD with the C-terminal effector-binding domain (EBD) or regulatory domain (RD; which comprises subdomains RD-I and RD-II). Here we show that the oligomeric structure of LeuO is a tetramer that binds with high affinity to DNA. A collection of single amino acid substitutions in the LeuO DBD indicated that this region is involved in oligomerization, in positive and negative regulation, as well as in DNA binding. Mutants with point mutations in the central and C-terminal regions of RD-I were affected in transcriptional activation. Deletion of the RD-II and RD-I C-terminal subdomains affected not only oligomerization but also DNA interaction, showing that they are involved in positive and negative regulation. Together, these data demonstrate that not only the C terminus but also the DBD of LeuO is involved in oligomer formation; therefore, each LeuO domain appears to act synergistically to maintain its regulatory functions in Salmonella enterica serovar Typhi.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.