ArticleBiology open2014
Various applications of TALEN- and CRISPR/Cas9-mediated homologous recombination to modify the Drosophila genome.
Article in Biology open, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 77 citations in OpenAlex.
- Highly frequent undesired insertional mutagenesis during Drosophila genome editing.PLoS genetics · 2026Article
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- A drug stabilizable GAL80Scientific reports · 2024Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- A CRISPR way for accelerating cereal crop improvement: Progress and challenges.Frontiers in genetics · 2022Review
- Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences.Biotech (Basel (Switzerland)) · 2021Review
- Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- ERp44/CG9911 promotes fat storage inAging · 2021Article
- Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife.Synthetic biology (Oxford, England) · 2020Review
- Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach.Biological procedures online · 2020Article
- Long noncoding RNA SMRG regulates Drosophila macrochaetes by antagonizing scute through E(spl)mβ.RNA biology · 2019Article
- Review
- The genome of the Hi5 germ cell line fromeLife · 2018Article
- Engineering the Drosophila Genome for Developmental Biology.Journal of developmental biology · 2017Review
- High-efficiency non-mosaic CRISPR-mediated knock-in and indel mutation in F0Development (Cambridge, England) · 2017Article
- The SWI/SNF Complex Protein Snr1 Is a Tumor Suppressor inCancer research · 2017Article
- Article
- TALEN-mediated genome-editing approaches in the liverwortPlant methods · 2017Article
- Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods.Frontiers in physiology · 2017Review
- Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion.G3 (Bethesda, Md.) · 2016Article
Corrections and comments
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Authors and funding
18 authors at 6 institutions in 2 countries.
Funding
Abstract
Modifying the genomes of many organisms is becoming as easy as manipulating DNA in test tubes, which is made possible by two recently developed techniques based on either the customizable DNA binding protein, TALEN, or the CRISPR/Cas9 system. Here, we describe a series of efficient applications derived from these two technologies, in combination with various homologous donor DNA plasmids, to manipulate the Drosophila genome: (1) to precisely generate genomic deletions; (2) to make genomic replacement of a DNA fragment at single nucleotide resolution; and (3) to generate precise insertions to tag target proteins for tracing their endogenous expressions. For more convenient genomic manipulations, we established an easy-to-screen platform by knocking in a white marker through homologous recombination. Further, we provided a strategy to remove the unwanted duplications generated during the "ends-in" recombination process. Our results also indicate that TALEN and CRISPR/Cas9 had comparable efficiency in mediating genomic modifications through HDR (homology-directed repair); either TALEN or the CRISPR/Cas9 system could efficiently mediate in vivo replacement of DNA fragments of up to 5 kb in Drosophila, providing an ideal genetic tool for functional annotations of the Drosophila genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.