ArticlePloS one2014
Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- Expanding the epidemiological insight: the role of synanthropic waterfowl in environmental transmission of trichomonads and acanthamoebae.Parasitology research · 2025Article
- First genetic characterisation and phylogenetic analysis of Trichomonadida from Gallus gallus domesticus (Aves: Phasianidae) and its nematode parasite Heterakis gallinarum (Ascaridida: Heterakidae) in Tunisia.Folia parasitologica · 2025Article
- Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single-Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as 'Non-Pathogenic'.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Review
- Molecular evidence for trichomonads and acanthamoebae in cloacal samples of synanthropic waterfowl.Parasitology research · 2025Article
- 18S rRNA gene metabarcoding for investigation of gastrointestinal parasite diversity in great cormorants.Scientific reports · 2025Article
- Host-specific targets of Histomonas meleagridis antigens revealed by immunoprecipitation.Scientific reports · 2025Article
- Genetic diversity of Trichomonads from Milu deer (Elaphurus davidianus) in China.Parasite (Paris, France) · 2025Article
- Feed Composition and Isolate ofFrontiers in veterinary science · 2022Article
- Histomonosis in Poultry: A Comprehensive Review.Frontiers in veterinary science · 2022Review
- Lethal infection caused by Tetratrichomonas gallinarum in black swans (Cygnus atratus).BMC veterinary research · 2021Article
- PCR and serology confirm the infection of turkey hens and their resilience to histomonosis in mixed flocks following high mortalities in toms.Parasites & vectors · 2019Article
- Article
- Molecular characterization of Histomonas meleagridis exoproteome with emphasis on protease secretion and parasite-bacteria interaction.PloS one · 2019Article
- Commensal or pathogen - a challenge to fulfil Koch's Postulates.British poultry science · 2017Article
- Histomonosis - an existing problem in chicken flocks in Poland.Veterinary research communications · 2015Article
- What is the importance of zoonotic trichomonads for human health?Trends in parasitology · 2014Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histomonas meleagridis is the aetiological agent of histomonosis or "blackhead disease". Histomonosis is of special importance today, because there is no effective treatment to prevent its occurrence with considerable losses for the poultry industry. Despite its importance only a few molecular studies have yet been performed to investigate the degree of genetic diversity between different isolates of this parasite. In the present study a collection of well defined samples, previously shown positive for the DNA of H. meleagridis, was used to investigate genetic relatedness of the parasite. Samples originated from 25 turkey flocks collected in France between 2007 and 2010. Additionally, diagnostic samples, collected at our Clinic in Vienna, from different European countries and Azerbaijan, during 2010 to 2013 were included in the analyses. For the analysis three different genetic loci were analyzed: 18S rRNA, α-actinin1 and rpb1 genes. To amplify partial sequences of α-actinin1 and rpb1 genes, primers specifically targeting H. meleagridis were designed. Following PCR, the sequences of 18S rRNA, α-actinin1 and rpb1 loci were analyzed. Phylogenetic analyses demonstrated separation of H. meleagridis isolates in two different clusters. The majority of isolates grouped within the cluster 1 and originated from different European countries. The cluster 2 was rare and predominantly found in samples originating from France. Considering that the genetic variability of clusters can be seen as two distinct genetic types we propose the term genotype instead of cluster.
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