ArticlePloS one2014
Acanthamoeba protease activity promotes allergic airway inflammation via protease-activated receptor 2.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Acanthamoeba profilin as a novel airway allergen with diverse sensitization patterns including pollen cross-reactivity.Parasites, hosts and diseases · 2026Article
- The Recombinant Profilin from Free-Living Amoebae Induced Allergic Immune Responses via TLR2.Journal of inflammation research · 2024Article
- Proteases of Acanthamoeba.Parasitology research · 2023Review
- Toll-like receptor 2 mediates Acanthamoeba-induced allergic airway inflammatory response in mice.PLoS neglected tropical diseases · 2023Article
- Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation.The Korean journal of parasitology · 2022Article
- Immunopathogenicity ofInternational journal of molecular sciences · 2021Review
- Identification of Free-Living Amoebas in Tap Water of Buildings with Storage Tanks in Korea.The Korean journal of parasitology · 2020Article
- Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models.Scientific reports · 2019Article
- Acanthamoeba profilin elicits allergic airway inflammation in mice.PLoS neglected tropical diseases · 2018Article
- Expression and Activity of COX-1 and COX-2 in Acanthamoeba sp.-Infected Lungs According to the Host Immunological Status.International journal of molecular sciences · 2018Article
- Protease-activated receptor 2 activates airway apical membrane chloride permeability and increases ciliary beating.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Culture supernatant of adipose stem cells can ameliorate allergic airway inflammation via recruitment of CD4Stem cell research & therapy · 2017Article
- A highly potent agonist to protease-activated receptor-2 reveals apical activation of the airway epithelium resulting in Ca2+-regulated ion conductance.American journal of physiology. Cell physiology · 2014Article
- Bronchopulmonary lophomoniasis: emerging disease or unsubstantiated legend?Parasites & vectors · 2014Article
- Analysis of differential expression of protease-activated receptors in patients with allergic fungal rhinosinusitis.Allergy & rhinology (Providence, R.I.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acanthamoeba is a free-living amoeba commonly present in the environment and often found in human airway cavities. Acanthamoeba possesses strong proteases that can elicit allergic airway inflammation. To our knowledge, the aeroallergenicity of Acanthamoeba has not been reported. We repeatedly inoculated mice with Acanthamoeba trophozoites or excretory-secretory (ES) proteins intra-nasally and evaluated symptoms and airway immune responses. Acanthamoeba trophozoites or ES proteins elicited immune responses in mice that resembled allergic airway inflammation. ES proteins had strong protease activity and activated the expression of several chemokine genes (CCL11, CCL17, CCL22, TSLP, and IL-25) in mouse lung epithelial cells. The serine protease inhibitor phenyl-methane-sulfonyl fluoride (PMSF) inhibited ES protein activity. ES proteins also stimulated dendritic cells and enhanced the differentiation of naive T cells into IL-4-secreting T cells. After repeated inoculation of the protease-activated receptor 2 knockout mouse with ES proteins, airway inflammation and Th2 immune responses were markedly reduced, but not to basal levels. Furthermore, asthma patients had higher Acanthamoeba-specific IgE titers than healthy controls and we found Acanthamoeba specific antigen from house dust in typical living room. Our findings suggest that Acanthamoeba elicits allergic airway symptoms in mice via a protease allergen. In addition, it is possible that Acanthamoeba may be one of the triggers human airway allergic disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.