ArticlePloS one2014
Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- Phenotype stability and dynamics of transposable elements in a strain of the microalga Tisochrysis lutea with improved lipid traits.PloS one · 2023Article
- Application of Microalgal Stress Responses in Industrial Microalgal Production Systems.Marine drugs · 2021Review
- Mixotrophic growth of the extremophile Galdieria sulphuraria reveals the flexibility of its carbon assimilation metabolism.The New phytologist · 2021Article
- Adaptive Laboratory Evolution of Microalgae: A Review of the Regulation of Growth, Stress Resistance, Metabolic Processes, and Biodegradation of Pollutants.Frontiers in microbiology · 2021Review
- Systems Biology of Cold Adaptation in the Polyextremophilic Red AlgaFrontiers in microbiology · 2019Article
- Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation.Biotechnology for biofuels · 2018Review
- Isolation, phenotypic characterization and genome wide analysis of aBiotechnology for biofuels · 2017Article
- Microfluidic high-throughput selection of microalgal strains with superior photosynthetic productivity using competitive phototaxis.Scientific reports · 2016Article
- The role of pyruvate hub enzymes in supplying carbon precursors for fatty acid synthesis in photosynthetic microalgae.Photosynthesis research · 2015Article
- Establishing Chlamydomonas reinhardtii as an industrial biotechnology host.The Plant journal : for cell and molecular biology · 2015Review
- Transcriptional program for nitrogen starvation-induced lipid accumulation in Chlamydomonas reinhardtii.Biotechnology for biofuels · 2015Article
- Article
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Authors and funding
7 authors.
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Abstract
Improving feedstock is critical to facilitate the commercial utilization of algae, in particular in open pond systems where, due to the presence of competitors and pests, high algal growth rates and stress tolerance are beneficial. Here we raised laboratory cultures of the model alga Chlamydomonas reinhardtii under serial dilution to explore the potential of crop improvement using natural selection. The alga was evolved for 1,880 generations in liquid medium under continuous light (EL population). At the end of the experiment, EL cells had a growth rate that was 35% greater than the progenitor population (PL). The removal of acetate from the medium demonstrated that EL growth enhancement largely relied on efficient usage of this organic carbon source. Genome re-sequencing uncovered 1,937 polymorphic DNA regions in the EL population with 149 single nucleotide polymorphisms resulting in amino acid substitutions. Transcriptome analysis showed, in the EL population, significant up regulation of genes involved in protein synthesis, the cell cycle and cellular respiration, whereas the DNA repair pathway and photosynthesis were down regulated. Like other algae, EL cells accumulated neutral lipids under nitrogen depletion. Our work demonstrates transcriptome and genome-wide impacts of natural selection on algal cells and points to a useful strategy for strain improvement.
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