ArticlePloS one2014
An alternative suite of universal primers for genotyping in multiplex PCR.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- First molecular record of Panaeolus subbalteatus (Psathyrellaceae, Agaricales) from Pakistan.Antonie van Leeuwenhoek · 2026Article
- Population status and genetic assessment of mugger (Crocodylus palustris) in a tropical regulated river system in North India.Scientific reports · 2024Article
- Population genetics of the critically endangered three-striped turtle, Batagur dhongoka, from the Ganga river system using mitochondrial DNA and microsatellite analysis.Scientific reports · 2024Article
- MultiplexSSR: A pipeline for developing multiplex SSR-PCR assays from resequencing data.Ecology and evolution · 2020Article
- Development of a Novel Phenotypic Roadmap to Improve Blueberry Quality and Storability.Frontiers in plant science · 2020Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The universal primer three-primer approach can dramatically reduce the cost when genotyping the microsatellites. One former research reported four universal primers that can be used in singleplex and multiplex genotyping. In this study, we proposed an alternative suite of universal primers with four dyes for genotyping 8-12 loci in one single run. This multiplex method was tested on Tetranychus truncatus. Published microsatellite loci of T. kanzawai, Frankliniella occidentalis and Nilaparvata lugens were modified as needed and also tested. The robustness of the method was confirmed by comparing with singleplex using multiple fluorophores and genotyping two populations of T. truncatus. This method showed lower signal strength than the singleplex three-primer system, but it was still sufficient to determine the fragment length. The cost of such a project can be reduced dramatically when many loci of different species are involved. In this way, laboratories performing population genetic analyses or studying several different species may benefit from the use of this cost-effective protocol.
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