ArticlePloS one2014
Piwi is required in multiple cell types to control germline stem cell lineage development in the Drosophila ovary.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 85 citations in OpenAlex.
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- The E-cadherin-Wnt-mir-994 Axis Repurposes a Cadherin Switch for Niche Robustness and Germline Stem Cell Maintenance.Cell proliferation · 2026Article
- Stem cell competition.Current topics in developmental biology · 2026Review
- The Role of Ovarian Granulosa Cells Related-ncRNAs in Ovarian Dysfunctions: Mechanism Research and Clinical Exploration.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- piRNAs as Potential Regulators of Mammary Gland Development and Pathology in Livestock.Veterinary sciences · 2025Review
- Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression.PLoS biology · 2025Article
- Non-gonadal expression of piRNAs is widespread across Arthropoda.FEBS letters · 2025Review
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- Gap junction-transported cAMP from the niche controls stem cell progeny differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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- New molecular targets in Hodgkin and Reed-Sternberg cells.Frontiers in immunology · 2023Review
- Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Novel intrinsic factor Yun maintains female germline stem cell fate through Thickveins.Stem cell reports · 2022Article
- Stonewall prevents expression of ectopic genes in the ovary and accumulates at insulator elements in D. melanogaster.PLoS genetics · 2022Article
- Primordial Germ Cell Development in the Poeciliid,Frontiers in cell and developmental biology · 2022Article
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- Multiple Niche Compartments Orchestrate Stepwise Germline Stem Cell Progeny Differentiation.Current biology : CB · 2021Article
- Ovarian somatic Piwi regulates nurse cell proliferation and oocyte specification inmicroPublication biology · 2020Article
- Piwi in the stem cell niche regulates nurse cell number and oocyte specification.microPublication biology · 2020Article
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Authors and funding
22 authors at 6 institutions in 2 countries.
Funding
Abstract
The piRNA pathway plays an important role in maintaining genome stability in the germ line by silencing transposable elements (TEs) from fly to mammals. As a highly conserved piRNA pathway component, Piwi is widely expressed in both germ cells and somatic cells in the Drosophila ovary and is required for piRNA production in both cell types. In addition to its known role in somatic cap cells to maintain germline stem cells (GSCs), this study has demonstrated that Piwi has novel functions in somatic cells and germ cells of the Drosophila ovary to promote germ cell differentiation. Piwi knockdown in escort cells causes a reduction in escort cell (EC) number and accumulation of undifferentiated germ cells, some of which show active BMP signaling, indicating that Piwi is required to maintain ECs and promote germ cell differentiation. Simultaneous knockdown of dpp, encoding a BMP, in ECs can partially rescue the germ cell differentiation defect, indicating that Piwi is required in ECs to repress dpp. Consistent with its key role in piRNA production, TE transcripts increase significantly and DNA damage is also elevated in the piwi knockdown somatic cells. Germ cell-specific knockdown of piwi surprisingly causes depletion of germ cells before adulthood, suggesting that Piwi might control primordial germ cell maintenance or GSC establishment. Finally, Piwi inactivation in the germ line of the adult ovary leads to gradual GSC loss and germ cell differentiation defects, indicating the intrinsic role of Piwi in adult GSC maintenance and differentiation. This study has revealed new germline requirement of Piwi in controlling GSC maintenance and lineage differentiation as well as its new somatic function in promoting germ cell differentiation. Therefore, Piwi is required in multiple cell types to control GSC lineage development in the Drosophila ovary.
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