ArticleActa biomaterialia2014
Effects of hydroxyapatite on endothelial network formation in collagen/fibrin composite hydrogels in vitro and in vivo.
Article in Acta biomaterialia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Preliminary Formulation-Dependent Angiogenesis-Related and Early Osteogenic Responses to Three-Dimensional Bioprinted Hydroxyapatite-Acrylated Palm Olein Scaffolds: An In Vitro Study.International journal of molecular sciences · 2026Article
- Directional bone matrix mineralization in the CAM assay is governed by vascular integration and matrix remodeling.bioRxiv : the preprint server for biology · 2026Article
- Construction of Vascularized Intestinal Organoids Based on Scaffolds, Hydrogels, and 3D Printing Technologies and Their Applications in Drug Delivery.International journal of nanomedicine · 2026Review
- A Vascularized Microphysiological System Reproducing Endochondral Ossification in Vitro to Study Ewing Sarcoma Proliferation and Migration.Advanced functional materials · 2026Article
- Collagen I Microfiber Promotes Brain Capillary Network Formation in Three-Dimensional Blood-Brain Barrier Microphysiological Systems.Biomedicines · 2024Article
- 3D printed elastomeric biomaterial mitigates compaction during in vitro vasculogenesis.Acta biomaterialia · 2023Article
- Endothelialization of Whey Protein Isolate-Based Scaffolds for Tissue Regeneration.Molecules (Basel, Switzerland) · 2023Article
- Coculture of Endothelial and Stromal Cells to Promote Concurrent Osteogenesis and Vasculogenesis.Tissue engineering. Part A · 2021Article
- Induced Pluripotent Stem Cell-Derived Endothelial Networks Accelerate Vascularization But Not Bone Regeneration.Tissue engineering. Part A · 2021Article
- Applications of nanomaterials in tissue engineering.RSC advances · 2021Review
- Characterization of Tissue Engineered Endothelial Cell Networks in Composite Collagen-Agarose Hydrogels.Gels (Basel, Switzerland) · 2020Article
- Spatiotemporally-controlled transgene expression in hydroxyapatite-fibrin composite scaffolds using high intensity focused ultrasound.Biomaterials · 2019Article
- 3D Microfluidic Bone Tumor Microenvironment Comprised of Hydroxyapatite/Fibrin Composite.Frontiers in bioengineering and biotechnology · 2019Article
- Compression-induced structural and mechanical changes of fibrin-collagen composites.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Fibrin mechanical properties and their structural origins.Matrix biology : journal of the International Society for Matrix Biology · 2017Review
- Bone biomaterials and interactions with stem cells.Bone research · 2017Review
- Microscale characterization of the viscoelastic properties of hydrogel biomaterials using dual-mode ultrasound elastography.Biomaterials · 2016Article
- Biomaterial-mediated strategies targeting vascularization for bone repair.Drug delivery and translational research · 2016Review
- Use of Hydroxyapatite Doping to Enhance Responsiveness of Heat-Inducible Gene Switches to Focused Ultrasound.Ultrasound in medicine & biology · 2016Article
- Enzymatic regulation of functional vascular networks using gelatin hydrogels.Acta biomaterialia · 2015Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Co-culture of endothelial cells (EC) and mesenchymal stem cells (MSC) results in robust vascular network formation in constrained 3-D collagen/fibrin (COL/FIB) composite hydrogels. However, the ability to form endothelial networks is lost when such gels are allowed to compact via cell-mediated remodeling. In this study, we created co-cultures of human EC and human MSC in both constrained and unconstrained COL/FIB matrices and systematically added nanoparticulate hydroxyapatite (HA, 0-20 mg ml(-1)), a bone-like mineral that has been shown to have pro-vasculogenic effects. Constructs cultured for 7 days were assayed for gel compaction, vascular network formation, and mechanical properties. In vitro, robust endothelial network formation was observed in constrained COL/FIB constructs without HA, but this response was significantly inhibited by addition of 5, 10, or 20 mg ml(-1) HA. In unconstrained matrices, network formation was abolished in pure COL/FIB constructs but was rescued by 1.25 or 2.5 mg ml(-1) HA, while higher levels again inhibited vasculogenesis. HA inhibited gel compaction in a dose-dependent manner, which was not correlated to endothelial network formation. HA affected initial stiffness of the gels, but gel remodeling abrogated this effect. Subcutaneous implantation of COL/FIB with 0, 2.5 or 2 0mg ml(-1) HA in the mouse resulted in increased perfusion at the implant site, with no significant differences between materials. Histology at day 7 showed both host and human CD31-stained vasculature infiltrating the implants. These findings are relevant to the design of materials and scaffolds for orthopedic tissue engineering, where both vasculogenesis and formation of a mineral phase are required for regeneration.
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Registered trials
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