ArticleBMC infectious diseases2014
Quantitative data from the SeptiFast real-time PCR is associated with disease severity in patients with sepsis.
Article in BMC infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Clinical diagnostic performance of droplet digital PCR for pathogen detection in patients with Escherichia coli bloodstream infection: a prospective observational study.BMC infectious diseases · 2025Observational
- Prognostic value of poly-microorganisms detected by droplet digital PCR and pathogen load kinetics in sepsis patients: a multi-center prospective cohort study.Microbiology spectrum · 2024Article
- Pathogen load and species monitored by droplet digital PCR in patients with bloodstream infections: A prospective case series study.BMC infectious diseases · 2022Article
- Performance of PCR/Electrospray Ionization-Mass Spectrometry on Whole Blood for Detection of Bloodstream Microorganisms in Patients with Suspected Sepsis.Journal of clinical microbiology · 2020Article
- The challenge of molecular diagnosis of bloodstream infections.World journal of microbiology & biotechnology · 2019Review
- 16S rDNA droplet digital PCR for monitoring bacterial DNAemia in bloodstream infections.PloS one · 2019Article
- Bacterial DNA patterns identified using paired-end Illumina sequencing of 16S rRNA genes from whole blood samples of septic patients in the emergency room and intensive care unit.BMC microbiology · 2018Article
- Emerging Technologies for Molecular Diagnosis of Sepsis.Clinical microbiology reviews · 2018Review
- Evaluation of a Commercial Multiplex PCR Assay for Detection of Pathogen DNA in Blood from Patients with Suspected Sepsis.PloS one · 2016Article
- Article
- Empiric antimicrobial therapy in severe sepsis and septic shock: optimizing pathogen clearance.Current infectious disease reports · 2015Article
- PCR assay for pathogen detection in ventriculostomy-related meningitis in neurosurgery patients: unanswered questions?Infection · 2015Article
- Development of a new real-time PCR system for simultaneous detection of bacteria and fungi in pathological samples.International journal of clinical and experimental pathology · 2015Article
- Nucleic acid-based methods for early detection of sepsis.Annals of cardiac anaesthesiaArticle
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5 authors.
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Abstract
backgroundThe commercial test, SeptiFast, is designed to detect DNA from bacterial and fungal pathogens in whole blood. The method has been found to be specific with a high rule-in value for the early detection of septic patients. The software automatically provides information about the identified pathogen, without quantification of the pathogen. However, it is possible to manually derive Crossing point (Cp) values, i.e. the PCR cycle at which DNA is significantly amplified. The aim of this study was to find out whether Cp values correlate to disease severity.
methodsWe used a study cohort of patients with positive results from SeptiFast tests for bacteria from a recent study which included patients with suspected sepsis in the Emergency department. Cp values were compared with disease severity, classified as severe sepsis/septic shock or non-severe sepsis, according to the criteria of the American College of Chest Physicians/Society of Critical Care Medicine.
resultsNinety-four patients were included. The prevalence of severe sepsis/septic shock in the study was 29%. SeptiFast positive tests from patients with severe sepsis/septic shock had significantly lower Cp values compared with those from patients with non-severe sepsis, median 16.9 (range: 7.3-24.3) versus 20.9 (range: 8.5-25.0), p < 0.001. Positive predictive values from the SeptiFast test for identifying severe sepsis/septic shock were 34% at Cp cut-off <25.0, 35% at Cp cut-off <22.5, 50% at Cp cut-off <20.0, and 73% at Cp cut-off <17.5. Patients with a positive Septifast test with a Cp value <17.5 had significantly more severe sepsis/septic shock (73% versus 15%, p < 0.001), were more often admitted to the Intensive Care Unit (23% versus 4%, p = 0.016), had positive blood culture (BC) more frequently (100% versus 32%, p < 0.001) and had longer hospital stays (median 19.5 [range: 4-78] days versus 5 [range: 0-75] days, p < 0.001) compared with those with a Cp value >17.5.
conclusionsOur results suggest that introducing quantitative data to the SeptiFast test could be of value in assessing sepsis severity. Moreover, such data might also be useful in predicting a positive BC result.
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