ArticleMolecular cell2014
Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes.
Article in Molecular cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 58 citations in OpenAlex.
- Oligo(uridine) tailing and exonucleolytic decay drive 40S ribosome degradation in response to stress.Molecular cell · 2026Article
- Article
- Mechanistic insights into recruitment and regulation of the RNA helicase UPF1 in replication-dependent histone mRNA decay.Nature communications · 2026Article
- Unraveling Histone Loss in Aging and Senescence.Cells · 2024Review
- Uridylation of the histone mRNA stem-loop weakens binding interactions with SLBP while maintaining interactions with 3'hExo.RNA biology · 2023Article
- Measuring the tail: Methods for poly(A) tail profiling.Wiley interdisciplinary reviews. RNA · 2023Review
- Knockouts of TUT7 and 3'hExo show that they cooperate in histone mRNA maintenance and degradation.RNA (New York, N.Y.) · 2022Article
- mRNA vaccine with unmodified uridine induces robust type I interferon-dependent anti-tumor immunity in a melanoma model.Frontiers in immunology · 2022Article
- A tale of non-canonical tails: gene regulation by post-transcriptional RNA tailing.Nature reviews. Molecular cell biology · 2020Review
- Mammalian RNA Decay Pathways Are Highly Specialized and Widely Linked to Translation.Molecular cell · 2020Article
- Stop codon context influences genome-wide stimulation of termination codon readthrough by aminoglycosides.eLife · 2020Article
- Determining degradation intermediates and the pathway of 3' to 5' degradation of histone mRNA using high-throughput sequencing.Methods (San Diego, Calif.) · 2019Review
- Role of oligouridylation in normal metabolism and regulated degradation of mammalian histone mRNAs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- The role of 3' end uridylation in RNA metabolism and cellular physiology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- NMD-degradome sequencing reveals ribosome-bound intermediates with 3'-end non-templated nucleotides.Nature structural & molecular biology · 2018Article
- Article
- Lost in Translation: Ribosome-Associated mRNA and Protein Quality Controls.Frontiers in genetics · 2018Review
- A new layer of rRNA regulation by small interference RNAs and the nuclear RNAi pathway.RNA biology · 2017Review
- Birth and Death of Histone mRNAs.Trends in genetics : TIG · 2017Review
- Loss of the RNA helicase SKIV2L2 impairs mitotic progression and replication-dependent histone mRNA turnover in murine cell lines.RNA (New York, N.Y.) · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Histone mRNAs are rapidly degraded when DNA replication is inhibited during S phase with degradation initiating with oligouridylation of the stem loop at the 3' end. We developed a customized RNA sequencing strategy to identify the 3' termini of degradation intermediates of histone mRNAs. Using this strategy, we identified two types of oligouridylated degradation intermediates: RNAs ending at different sites of the 3' side of the stem loop that resulted from initial degradation by 3'hExo and intermediates near the stop codon and within the coding region. Sequencing of polyribosomal histone mRNAs revealed that degradation initiates and proceeds 3' to 5' on translating mRNA and that many intermediates are capped. Knockdown of the exosome-associated exonuclease PM/Scl-100, but not the Dis3L2 exonuclease, slows histone mRNA degradation consistent with 3' to 5' degradation by the exosome containing PM/Scl-100. Knockdown of No-go decay factors also slowed histone mRNA degradation, suggesting a role in removing ribosomes from partially degraded mRNAs.
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