ArticleBiophysical journal2014
Adipocyte stiffness increases with accumulation of lipid droplets.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasion.Cell biomaterials · 2026Article
- The Mechanobiology of Adipocyte Hypertrophy: Cytoskeletal Remodelling as a Driver of Insulin Resistance in Obesity.Cell biochemistry and function · 2026Review
- Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasion.bioRxiv : the preprint server for biology · 2025Article
- Towards linking histological changes to liver viscoelasticity: a hybrid analytical-computational micromechanics approach.Physics in medicine and biology · 2025Article
- Polyunsaturated fatty acids suppress PIEZO ion channel mechanotransduction in articular chondrocytes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Article
- Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability.Nature communications · 2024Article
- Atomic force microscopy characterization of white and beige adipocyte differentiation.In vitro cellular & developmental biology. Animal · 2024Article
- Mechanobiology of Adipocytes.Biology · 2024Review
- Cytoskeletal rearrangement precedes nucleolar remodeling during adipogenesis.Communications biology · 2024Article
- Small lipid droplets are rigid enough to indent a nucleus, dilute the lamina, and cause rupture.The Journal of cell biology · 2023Article
- Local fat content determines global and local stiffness in livers with simple steatosis.FASEB bioAdvances · 2023Article
- Adipose-targeted SWELL1 deletion exacerbates obesity- and age-related nonalcoholic fatty liver disease.JCI insight · 2023Article
- Advanced Glycation End Products Effects on Adipocyte Niche Stiffness and Cell Signaling.International journal of molecular sciences · 2023Article
- Light distribution in fat cell layers at physiological temperatures.Scientific reports · 2023Article
- Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling.International journal of molecular sciences · 2022Article
- Adipose Tissue Development Relies on Coordinated Extracellular Matrix Remodeling, Angiogenesis, and Adipogenesis.Biomedicines · 2022Review
- Effect of Lauric vs. Oleic Acid-Enriched Diets on Leptin Autoparacrine Signalling in Male Mice.Biomedicines · 2022Article
- Review
- Oleic Acid Protects Endothelial Cells from Silica-Coated Superparamagnetic Iron Oxide Nanoparticles (SPIONs)-Induced Oxidative Stress and Cell Death.International journal of molecular sciences · 2022Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Adipogenesis and increase in fat tissue mass are mechanosensitive processes and hence should be influenced by the mechanical properties of adipocytes. We evaluated subcellular effective stiffnesses of adipocytes using atomic force microscopy (AFM) and interferometric phase microscopy (IPM), and we verified the empirical results using finite element (FE) simulations. In the AFM studies, we found that the mean ratio of stiffnesses of the lipid droplets (LDs) over the nucleus was 0.83 ± 0.14, from which we further evaluated the ratios of LDs over cytoplasm stiffness, as being in the range of 2.5 to 8.3. These stiffness ratios, indicating that LDs are stiffer than cytoplasm, were verified by means of FE modeling, which simulated the AFM experiments, and provided good agreement between empirical and model-predicted structural behavior. In the IPM studies, we found that LDs mechanically distort their intracellular environment, which again indicated that LDs are mechanically stiffer than the surrounding cytoplasm. Combining these empirical and simulation data together, we provide in this study evidence that adipocytes stiffen with differentiation as a result of accumulation of LDs. Our results are relevant to research of adipose-related diseases, particularly overweight and obesity, from a mechanobiology and cellular mechanics perspectives.
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